Rabbit staphylocosis and bordetellosis duplex tissue inactivated vaccine and preparation method

A technology for rabbit staphylococcal disease and rabbit Bordetella disease, applied in the biological field, can solve the problems of economic loss in the rabbit industry and achieve the effect of reducing the number of immunizations

Inactive Publication Date: 2018-11-02
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Rabbit staphylococcal disease and Bordetella disease are common diseases in the rabbit breeding process, causing serious economic losses to the rabbit industry

Method used

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  • Rabbit staphylocosis and bordetellosis duplex tissue inactivated vaccine and preparation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Preparation of Rabbit Staphylococcal Disease Tissue Inactivated Antigen Solution

[0032] 1. Rabbit staphylococcal disease disease material

[0033] Rabbits with natural onset, death or dying of Staphylococcus aureus infection were selected, and lesion tissues (trachea, lung, liver, spleen, mastitis tissue, foot dermatitis tissue, subcutaneous abscess tissue, other purulent lesions) were collected aseptically. tissue), remove the fat and connective tissue in the diseased tissue, weigh 100g of the diseased tissue, chop it up, add 200mL 4℃ pre-cooled sterilized saline, homogenize it in an ice bath, and use it now;

[0034] 2. Proliferation of Staphylococcus aureus

[0035] Select healthy rabbits weighing about 1.5kg, negative for Staphylococcus aureus antibody, and negative for Staphylococcus aureus, inoculate the tissue homogenate obtained in step 1 with intranasal drops (50 μL in each nostril), and inoculate the rabbits for 25 days in an isolator. When the inoculat...

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Abstract

The invention relates to a rabbit staphylocosis and bordetellosis duplex tissue inactivated vaccine and a preparation method thereof. The rabbit staphylocosis and bordetellosis duplex tissue inactivated vaccine consists of rabbit staphylocosis tissue inactivated positive control antigen and rabbit bordetellosis tissue inactivated positive antigen according to the volume ratio of 3: 1. The rabbit staphylocosis tissue inactivated positive control antigen is prepared from pathological tissues of a rabbit affected with the rabbit staphylocosis by homogenizing, filtering, centrifugation sedimeantation and inactivating; and the rabbit bordetellosis tissue inactivated positive antigen is prepared from pathological tissues of a rabbit affected with rabbit bordetellosis by homogenizing, filtering,centrifugation sedimeantation and inactivating. The duplex tissue inactivated vaccine is highly targeted and high in safety, and can effectively prevent rabbit staphylocosis and rabbit bordetellosis.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a dual tissue inactivated vaccine of rabbit staphylococcus disease and bordetella disease and a preparation method thereof. Background technique [0002] Rabbit staphylococcal disease is caused by Staphylococcus aureus ( Staphylococcus aureus ) is a multisymptomatic infectious disease caused by infection of rabbits. Clinically, the disease is mainly characterized by foot dermatitis, mastitis, subcutaneous abscess, and abscesses in various tissues and organs throughout the body, and respiratory symptoms can also be seen. Dead or near-death rabbits with respiratory symptoms showed extreme body weight loss and suppurative pneumonia. Rabbit staphylococcal disease occurs mainly in warm and humid seasons, and rabbits of different ages and breeds can be affected. [0003] Bordetella rabbits are caused by Bordetella bronchiseptica ( Bordetella bronchiseptica ) is a r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/116A61K39/02A61K39/085A61P31/04
CPCA61K39/0208A61K39/085A61P31/04A61K2039/70A61K2039/521A61K2039/552
Inventor 王锦祥谢喜平陈岩锋桑雷孙世坤陈冬金
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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