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Phyllostachys heterocycla bamboo shoot shell arabinogalactan as well as preparation and application thereof

A technology of arabinogalactan and arabinose, which is applied in the field of arabinogalactan and its preparation from moso bamboo shoot shells, achieving the effects of low equipment requirements, easy control, and low cost

Active Publication Date: 2018-11-02
ZHEJIANG FORESTRY ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the above-mentioned reports are about the primary structure of bamboo polysaccharides, and do not involve the advanced structure analysis of this type of polysaccharides, such as its monosaccharide composition, glycosidic bond connection mode, etc.; more and more studies have shown that polysaccharides are important. The performance of the function is determined by its structural characteristics. Its higher-level structure (secondary and tertiary structures) is closer. The biological activity of polysaccharides is closely related to its molecular weight and molecular chain conformation. It is more important to understand the conformation of the sugar molecule. Help to elucidate the mechanism of action of its biological activity

Method used

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  • Phyllostachys heterocycla bamboo shoot shell arabinogalactan as well as preparation and application thereof
  • Phyllostachys heterocycla bamboo shoot shell arabinogalactan as well as preparation and application thereof
  • Phyllostachys heterocycla bamboo shoot shell arabinogalactan as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] (1) Pretreatment: Fresh bamboo shoot shells are cleaned with tap water, dried in an oven at 55°C for 4 hours, then crushed, and passed through an 80-mesh sieve to obtain the bamboo shoot shell powder for use.

[0055] (2) Extraction: Add distilled water to the moso bamboo shoot shell powder in step (1) to form a feed solution, extract at 95°C, centrifuge, and concentrate the resulting water extract to obtain a concentrated solution, and then add 4 times the volume and volume of the concentrated solution The percent concentration of 95% ethanol aqueous solution was stirred and mixed, precipitated overnight at 2°C, centrifuged, and the precipitate obtained by centrifugation was taken to obtain a crude polysaccharide of Phyllostachys edulis shoot shell.

[0056] (3) Deproteinization: The aqueous solution of crude bamboo shoot husk polysaccharide obtained in step (2) is hydrolyzed with papain in a 55℃ water bath for 2 hours. The weight of papain is 1.5% of the weight of the crude...

Embodiment 2

[0061] (1) Pretreatment: The fresh bamboo shoot shells are cleaned with tap water, dried in an oven at 50°C for 5 hours, then crushed, and passed through a 150-mesh sieve to obtain the bamboo shoot shell powder for use.

[0062] (2) Extraction: add distilled water to the moso bamboo shoot shell powder in step (1) to form a feed solution, extract at 75°C, centrifuge, and concentrate the resulting aqueous extract to obtain a concentrated solution, and then add 4 times the volume and volume of the concentrated solution A 96% ethanol aqueous solution with a concentration of 96% was stirred and mixed well, precipitated overnight at 3°C, centrifuged, and the precipitate obtained by centrifugation was taken to obtain a crude polysaccharide of Phyllostachys edulis shoot shell.

[0063] (3) Deproteinization: The aqueous solution of crude bamboo shoot husk polysaccharide obtained in step (2) is enzymatically hydrolyzed with papain in a water bath at 50°C for 2.5 hours. The weight of papain is...

Embodiment 3

[0068] (1) Pretreatment: The fresh bamboo shoot shells are cleaned with tap water, dried in an oven at 60°C for 3 hours, then crushed, and passed through a 120-mesh sieve to obtain the bamboo shoot shell powder for use.

[0069] (2) Extraction: Add distilled water to the moso bamboo shoot shell powder in step (1) to form a feed solution, extract at 85°C, centrifuge, and concentrate the resulting water extract to obtain a concentrated solution. Then add 5 times the volume and volume of the concentrated solution. A 90% ethanol aqueous solution with a percent concentration was stirred and mixed well, precipitated overnight at 5°C, centrifuged, and the precipitate obtained by centrifugation was taken to obtain a crude polysaccharide of Phyllostachys edulis shoot shell.

[0070] (3) Deproteinization: The aqueous solution of crude bamboo shoot husk polysaccharide obtained in step (2) is enzymatically hydrolyzed with papain in a 55°C water bath for 2 hours. The weight of papain is 1% of th...

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Abstract

The invention discloses phyllostachys heterocycla bamboo shoot shell arabinogalactan as well as preparation and an application thereof. The phyllostachys heterocycla bamboo shoot shell arabinogalactanis prepared from 99% or more by weight of polysaccharide; the polysaccharide is prepared from galactose, arabinose, xylose and galacturonic acid in a mole ratio being (6.80-8.50):(5.50-7.30):(1.10-1.90):(0.70-1.30). The preparation method of the phyllostachys heterocycla bamboo shoot shell arabinogalactan comprises the following steps: extracting phyllostachys heterocycla bamboo shoot shell crudepolysaccharide by water extraction and alcohol precipitation, removing protein with an enzyme-Sevage combined method, performing dialysis, anion exchange chromatography and gel filtration chromatography for purification, and separating and purifying components by vacuum freeze drying to obtain the phyllostachys heterocycla bamboo shoot shell arabinogalactan. According to component analysis, structural identification and immune function research on the purified polysaccharide, the phyllostachys heterocycla bamboo shoot shell arabinogalactan has obvious hypoglycemic activity, can be used as a hypoglycemic functional product, can also be used for preparing a hypoglycemic functional product, and can be widely used in the fields of food, health care products, animal feed, medicine and the like.

Description

Technical field [0001] The invention relates to the field of plant polysaccharides, in particular to a moso bamboo shoot shell arabinogalactan and its preparation and use, belonging to the field of macromolecules and molecular biology. Background technique [0002] Phyllostachys heterocycla bamboo shoot (Phyllostachys heterocycla bamboo shoot) belongs to the bamboo subfamily of Gramineae. It is a fast-growing multifunctional forestry plant. It has a long history of cultivation and consumption in China, Japan and other Asian countries. It is widely used in building materials and furniture. , Paper, chopsticks and food sources. Bamboo shoots are sweet and slightly cold, and have biological effects such as antioxidant, liver protection, lowering blood sugar, lowering blood lipid, anti-inflammatory, anti-fatigue and regulating immunity. The freshly unearthed bamboo shoots each year are mainly processed into traditional bamboo shoot preservation food and flavored bamboo shoots. The u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 贺亮程俊文李琴张建刘焕燕魏海龙王衍彬
Owner ZHEJIANG FORESTRY ACAD
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