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A kind of excellent tomato variety in vitro culture and microbud grafting method

An in vitro culture, high-quality technology, applied in grafting, horticultural methods, botanical equipment and methods, etc., can solve the problems of high cost, premature aging of plants, and large amount of seedlings, so as to reduce drug costs, increase yield, cost saving effect

Active Publication Date: 2022-07-15
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The amount of seedlings used for soilless cultivation of tomato is large, the cost is high, and the plants are prone to premature aging, and the total output is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for in vitro culture and microbud grafting of stem sections of fine tomato varieties, comprising the following steps:

[0025] (1) Effective seedlings were obtained by in vitro induction of stem segments and 25-day proliferation culture of tomato varieties with good quality;

[0026] (2) Rootstock seedlings are obtained by inducing and culturing seeds of soil-borne disease-resistant tomato rootstock varieties for 25 days;

[0027] (3) Graft the effective seedlings on the rootstocks in the bottle, and after culturing, get the grafted seedlings with stable growth. After 7 days of natural light curing, the seedlings are cultivated in the greenhouse and then transplanted to the field for colonization.

[0028] Step (1) The specific cultivation method of effective seedlings is as follows:

[0029] 1) Material selection and disinfection: take out the tomato seeds and rinse them with running water, soak them in saturated bleaching powder supernatant for 15 minutes, a...

Embodiment 2

[0038] A method for in vitro culture and microbud grafting of stem sections of fine tomato varieties, comprising the following steps:

[0039] (1) To obtain effective seedlings by in vitro induction of stem segments and 20-day proliferation culture of tomato varieties with good quality;

[0040] (2) Rootstock seedlings are obtained by inducing and culturing seeds of soil-borne disease-resistant tomato rootstock varieties for 30 days;

[0041](3) Graft the effective seedlings on the rootstocks in the bottle, and after culturing, get the grafted seedlings with stable growth. After 7 days of natural light curing, the seedlings are cultivated in the greenhouse and then transplanted to the field for colonization.

[0042] Step (1) The specific cultivation method of effective seedlings is as follows:

[0043] 1) Material selection and disinfection: Take out the tomato seeds and rinse them with running water, soak them in saturated bleaching powder supernatant for 15 minutes, and br...

Embodiment 3

[0053] A method for in vitro culture and microbud grafting of stem sections of fine tomato varieties, comprising the following steps:

[0054] (1) To obtain effective seedlings by in vitro induction of stem segments and 22-day proliferation culture of tomato varieties with good quality;

[0055] (2) The seeds of tomato rootstock varieties with resistance to soil-borne diseases were induced and cultured for 28 days to obtain rootstock seedlings;

[0056] (3) Graft the effective seedlings on the rootstocks in the bottle, and after culturing, get the grafted seedlings with stable growth. After 7 days of natural light curing, the seedlings are cultivated in the greenhouse and then transplanted to the field for colonization.

[0057] Step (1) The specific cultivation method of effective seedlings is as follows:

[0058] 1) Material selection and disinfection: take out the tomato seeds and rinse them with running water, soak them in saturated bleaching powder supernatant for 15 min...

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PUM

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Abstract

The invention belongs to the field of agricultural production, in particular to a method for in vitro culture and microbud grafting of stem segments of fine tomato varieties. The method includes the following steps: (1) effective seedlings are obtained by in vitro induction and proliferation culture of selected tomato varieties with good quality through stem segments; (2) seeds of tomato rootstock varieties with resistance to soil-borne diseases are obtained by induction and culture Sterile rootstock seedlings; (3) grafting the effective seedlings on the rootstocks in the bottle, after culturing, the grafted seedlings with stable growth are obtained, after 7-10d natural light refining the seedlings, after 20-30d transplanting in the greenhouse, the transplanted seedlings can be transplanted. Planted in the field. Transplant into the field. The culture method has the advantages of low cost, high tomato yield, good quality, strong stress resistance and strong adaptability, and is convenient for large-scale popularization and application.

Description

technical field [0001] The invention belongs to the field of agricultural production, in particular to a method for in vitro culture and microbud grafting of stem segments of fine tomato varieties. Background technique [0002] Tomato is rich in protein, sugar, minerals and vitamins. It is rich in nutrients and delicious in flavor. It is one of the important crops in facility cultivation. Tomato can bloom and set fruit continuously. In production, using in vitro culture can effectively save costs, improve tomato utilization, and improve yield and quality. [0003] In southern my country, less arable land, serious soil-borne diseases, and continuous cropping obstacles restrict the development of tomato. The amount of seedlings used in soilless cultivation of tomato is large, the cost is high, and the plant is prone to premature senescence, and the total yield is not high. Therefore, there is a need for a method of in vitro culture and microbud grafting of stem sections of e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G2/30A01G31/00
CPCA01G2/30A01G31/00A01H4/001A01H4/008
Inventor 钟凤林何碧珠许茹王树彬尚春雨杜志杰闫星林义章李洪龙曹萍
Owner FUJIAN AGRI & FORESTRY UNIV