A living underwater adhesive material and its application
A composition, fusion protein technology, applied in the direction of adhesive types, polysaccharide adhesives, polymer adhesive additives, etc., can solve problems such as lack of integration and optimization
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[0074] Another aspect of the present invention provides the method for preparing the biofilm, comprising: cultivating the above-mentioned expression system under conditions suitable for forming the biofilm. The conditions for forming biofilms generally depend on the type of expression system used, and suitable conditions for forming biofilms should be known to those skilled in the art, for example, solid MSGG or MSGG liquid can be used to cultivate biofilms Methods.
[0075] Another aspect of the present invention provides the biofilm obtained by the above-mentioned expression system or the above-mentioned preparation method.
[0076] Another aspect of the present invention provides the use of the biofilm in the field of underwater adhesive preparation. The underwater adhesive can be an adhesive used for different substrate materials, for example, can be various applicable inorganic and / or organic substances, and the inorganic substances can include but not limited to inorgan...
Embodiment 1
[0095] Example 1: Modular design based on the viscosity characteristics of amyloid TasA and mussel fibroin
[0096] This example adopts the bacterial strain TD ( figure 1 d), which can minimize the impact of the biofilm's own viscosity on the modular design, and is also conducive to more effectively reflecting the contribution of the design to improving the biofilm viscosity. The present invention constructs the pHT01-tapA-sipW-tasA plasmid exogenously expressing amyloid TasA as a control ( figure 2 a), and the plasmid pHT01-tapA-sipW-tasA-mefp5 capable of simultaneously expressing amyloid protein and mussel silk protein ( figure 2 a).
[0097] The activated Escherichia coli TG1 (Biovector, BioVector1058 12-6) was picked from the LB plate, inoculated in 5 ml of LB medium, and cultured overnight at 37° C. with shaking. Inoculate the bacterial solution at a ratio of 1:100, take 250 μl of the bacterial solution in 25 ml of LB medium, and shake and culture at 37°C for 2-3 hou...
Embodiment 2
[0101] Embodiment 2: based on EPS and Fe 3+ Modular design of viscous features mediated by electrostatic interactions
[0102] EPS polysaccharide, as the main component of biofilm, is generally considered as a polyanionic polymer, through the introduction of transition metal ions (Fe 3+ ) to chelate it, it can further improve its internal cross-linking characteristics. This example adopts the TD bacterial strain as a control, adopts the DD bacterial strain ( figure 1 c), by comparing TD and DD biofilms on Fe 3+ The different reactions of EPS and Fe 3+ , and measure its effect on biofilm viscosity.
[0103] The above method for culturing biofilms with solid MSGG was adopted, and the viscoelasticity of biofilms was measured using a rheometer (Anton Paar MCR101). The measurement method and parameters are the same as the above-mentioned example 1. It can be seen that the polysaccharide EPS component produced, although the elasticity of the biofilm was not greatly improved, i...
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