Culture method of probiotics and application thereof

A culturing method and probiotic technology are applied in the field of probiotic culturing, which can solve the problems of difficulty in preservation of probiotics in the previous period, and achieve the effect of good heat resistance.

Active Publication Date: 2018-11-06
ZHONGKE YIKANG BEIJING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the above-mentioned technical problems, the present invention aims to provide a method for cultivating probiotics and its application. The technical problems to be solved are on the one hand the problem that t...

Method used

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  • Culture method of probiotics and application thereof
  • Culture method of probiotics and application thereof
  • Culture method of probiotics and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1 A highly active probiotic composition that improves the diversity of intestinal flora in infants and young children

[0064] Formula: comprise the following components by weight parts:

[0065] 40 parts of resistant dextrin, 8 parts of galacto-oligosaccharide, 10 parts of fructo-oligosaccharide, 8 parts of Bifidobacterium lactis HN019 freeze-dried powder, 4 parts of Bifidobacterium animalis BB-12 freeze-dried powder, Bifidobacterium breve M- 4 parts of 16V freeze-dried powder, 2 parts of Lactobacillus rhamnosus HN001 freeze-dried powder and 6 parts of Lactobacillus fermentum CECT5716 freeze-dried powder.

[0066] Preparation method: the specific steps include:

[0067] (1) Preparation of powder: pass the resistant dextrin, galacto-oligosaccharide and fructo-oligosaccharide through a 100-mesh sieve respectively, then mix the three kinds of powders according to the weight of the formula and stir repeatedly to obtain the mixed powder A, which is set aside;

[0...

Embodiment 2

[0074] Example 2 A highly active probiotic composition that improves the diversity of intestinal flora in infants and young children

[0075] Formula: comprise the following components by weight parts:

[0076]60 parts of resistant dextrin, 15 parts of galactooligosaccharide, 8 parts of fructooligosaccharide, 3 parts of Bifidobacterium lactis HN019 freeze-dried powder, 6 parts of Bifidobacterium animalis BB-12 freeze-dried powder, Bifidobacterium breve M- 6 parts of 16V freeze-dried powder, 6 parts of Lactobacillus rhamnosus HN001 freeze-dried powder and 4 parts of Lactobacillus fermentum CECT5716 freeze-dried powder.

[0077] Preparation method: the specific steps include: except that the content of each component is different, other operations and steps are the same as in Example 1.

Embodiment 3

[0078] Example 3 A highly active probiotic composition that improves the diversity of intestinal flora in infants and young children

[0079] Formula: comprise the following components by weight parts:

[0080] 50 parts of resistant dextrin, 12 parts of galacto-oligosaccharide, 12 parts of fructo-oligosaccharide, 8 parts of Bifidobacterium lactis HN019 freeze-dried powder, 4 parts of Bifidobacterium animalis BB-12 freeze-dried powder, Bifidobacterium breve M- 4 parts of 16V freeze-dried powder, 2 parts of Lactobacillus rhamnosus HN001 freeze-dried powder and 8 parts of Lactobacillus fermentum CECT5716 freeze-dried powder.

[0081] Preparation method: the specific steps include: except that the content of each component is different, other operations and steps are the same as in Example 1.

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Abstract

The invention discloses a culture method of probiotics. The method comprises the following steps: adding a porous material into a culture medium containing somatomedin for promoting reproduction of probiotics, ensuring that the volume ratio of the culture medium to the porous material is 1: 100, and inoculating the probiotics into the culture medium, so as to perform culture and realize probioticsculture. The obtained probiotics can be applied to products of preparation of food, food additives, medicine and feed or environmental protection treatment. The obtained probiotics has excellent heatresistance, acid resistance and drug resistance, and has a wide application prospect in the fields of food, medicine, feed and environmental protection.

Description

technical field [0001] The invention belongs to the technical field of probiotic cultivation and activation, and in particular relates to a cultivation method and application of probiotics. Background technique [0002] Probiotics are a class of active microorganisms that are beneficial to the host. They are a general term for active beneficial microorganisms that colonize the human intestinal tract and reproductive system and can produce definite health effects, thereby improving the host's micro-ecological balance and exerting beneficial effects. Supplementing probiotics can not only regulate the stomach, but also enhance human immunity, relieve lactose intolerance, prevent reproductive system infections, promote intestinal and digestive system health, lower serum cholesterol, help absorb nutrients, and create a healthy intestinal tract. Studies in recent years have shown that there are more than 1,000 kinds of bacteria in the human body, especially the bacteria in the gas...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/20A23L33/135A23L33/125A23K10/18A23K20/163C12R1/01C12R1/225
CPCA23K10/18A23K20/163A23L33/125A23L33/135C12N1/20C12N1/38A23V2002/00A23V2400/143A23V2400/515A23V2400/519A23V2400/175A23V2400/531A23V2200/3204A23V2250/28A23V2250/5114
Inventor 张烨刘海霞耿然
Owner ZHONGKE YIKANG BEIJING BIOTECH CO LTD
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