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Breeding method of alliumcepa male sterile lines

A male sterile line, onion technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, angiosperms/flowering plants, etc., can solve the problems of time-consuming and laborious, and achieve the effect of short breeding cycle and important application value

Active Publication Date: 2018-11-06
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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AI Technical Summary

Problems solved by technology

The traditional method is to continuously backcross 4-6 generations, but it takes more than 10 years, which is time-consuming and laborious

Method used

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  • Breeding method of alliumcepa male sterile lines
  • Breeding method of alliumcepa male sterile lines
  • Breeding method of alliumcepa male sterile lines

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Embodiment 1

[0030] Embodiment 1, utilizing protoplast asymmetric fusion technique to create onion male sterile line

[0031] 1. Obtaining the protoplasts of onion heterologous cytoplasmic male sterile line skiA and the protoplasts of Mesophytic Akadama

[0032] According to the literature (Chen Li, Liang Yi, Wang Liping, Tian Baohua. Separation and purification of onion callus protoplasts. China Agricultural Science Bulletin, 2011, 27(31): 147-151.) Separation and purification of onion heterologous cells Protoplasts of the male sterile line skiA. Specific steps are as follows:

[0033] 1. Take the plump and robust seeds of onion heterocytoplasmic male sterile line skiA, rinse them with sterile water for 10 minutes (the purpose is to remove surface impurities), then soak them with 75% (v / v) ethanol aqueous solution for 30 seconds, and finally use 0.1% HgCl 2 The solution was soaked for 10 minutes, and washed 5 times with sterile water.

[0034] 2. After completing step 1, take the seed...

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Abstract

The invention discloses a breeding method of alliumcepa male sterile lines. The breeding method is characterized by taking an alliumcepa cytoplasmic male sterile line as a donor and an alliumcepa selfing line as a receptor and carrying out asymmetric protoplast fusion, thus obtaining the alliumcepa male sterile lines. An experiment verifies that five alliumcepa male sterile lines are bred by adopting the method and utilizing an alliumcepa alloplasmic cytoplasmic male sterile line skiA and raw akadama, and the five alliumcepa male sterile lines are all in forms different from that of parents; plants are strong in growth vigor and are neutrophilous, bulb scarfskin is crimson, and no pollen is generated. The breeding method of the alliumcepa male sterile line, provided by the invention, is short in breeding period and has important application value.

Description

technical field [0001] The invention belongs to the field of plant breeding, and in particular relates to a method for breeding male sterile lines of onions. Background technique [0002] Onion (Alliumcepa) is an important worldwide and health-care vegetable, which occupies a very important position in the annual supply and export trade of vegetables in my country. The annual planting area of ​​onions in my country is more than 300,000 hectares, ranking second in the world, but due to reasons such as varieties, the yield per unit area ranks below 20. Due to the late start of onion variety breeding in my country, insufficient attention has been paid to it, and the long period of onion variety breeding, resulting in a large gap between domestic onion self-cultivation level and international advanced countries. For a long time, domestic onion hybrid seeds and even some conventional varieties have been monopolized by foreign seed companies or sourced from abroad. Imported seed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/05A01H1/02A01H5/00A01H6/56
CPCA01H1/02A01H5/00C12N15/02
Inventor 梁毅李晓杰
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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