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A kit for detecting the methylation of pax1 gene in cervical cancer

A kit and technology for cervical cancer, applied in the field of kits for detection of cervical cancer PAX1 gene methylation, can solve the problems of complex operation, cross-contamination of external environment and detection reagents, and inability to integrate, and achieve the effect of avoiding cross-contamination

Active Publication Date: 2022-04-12
GUANGZHOU HEAS BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The detection process is cumbersome, and many links need to be manually operated; nucleic acid extraction, nucleic acid sulfite conversion, and nucleic acid methylation detection are divided into three parts, which cannot be performed at the same time. There are many steps and complicated operations; nucleic acid extraction and nucleic acid sulfite conversion cannot be combined. The nucleic acid methylation detection reagents are integrated, and the detection process is open, which is easy to cause cross-contamination between the external environment and the detection reagents; the detection time is long

Method used

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  • A kit for detecting the methylation of pax1 gene in cervical cancer
  • A kit for detecting the methylation of pax1 gene in cervical cancer
  • A kit for detecting the methylation of pax1 gene in cervical cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] This embodiment provides a kit for detecting methylation of PAX1 gene in cervical cancer, such as Figure 1-7 As shown, it includes an extraction chamber 1 , a cracking mechanism 2 , a washing mechanism 3 , an elution mechanism 4 , a driving mechanism 5 , an amplification tube 6 , an upper cover 7 , a bracket 8 and a bottom cover 9 .

[0099] Among them, the extraction chamber 1 is made of elastic material, which includes a lysis mechanism 2, a washing mechanism 3, an elution mechanism 4, a buffer tank 101 and an elastic sealing film 104 distributed in sequence according to the flow direction of the sample; the bracket 8 is fixed on the extraction chamber 1. , the two are bonded by an adhesive; the upper cover 7 and the bottom cover 9 are detachably connected, the two enclose a cavity, and the extraction bin 1 and the bracket 8 are located in the cavity; the bracket 8 is provided with an upper cover mounting hole 801 , used to connect with the upper cover 7 .

[0100] ...

Embodiment 2

[0151] This embodiment provides a kit for detecting methylation of PAX1 gene in cervical cancer, such as Figure 8-14 As shown, it includes an extraction chamber 21 , a lysis mechanism 22 , a washing mechanism 23 , an elution mechanism 24 , a driving mechanism 25 , an amplification tube 26 and a top cover 27 .

[0152] The extraction chamber 21 includes a lysis mechanism 22 , a washing mechanism 23 , an elution mechanism 24 and a buffer tank 2101 , which are sequentially distributed according to the flow direction of the sample.

[0153] The cracking mechanism 22 is provided with a cracking reagent and a magnetic substance; the magnetic substance is a magnetic bead.

[0154] The washing mechanism 23 includes a first oil tank 2301 and a washing tank 2302 , and the washing tank 2302 is provided with a washing liquid; the first oil tank 2301 is located between the cracking mechanism 22 and the washing tank 2302 .

[0155] The elution mechanism 24 includes a second oil tank 2401 ...

Embodiment 3

[0169] The difference between this example and Example 1 is that the amplification reaction is performed in the buffer tank without setting an amplification tube; at this time, there is no need to set a connector, and a discharge port is directly set on the buffer tank for amplification Increase the efflux of the sample after the reaction.

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Abstract

The invention provides a kit for detecting the methylation of cervical cancer PAX1 gene, which is provided with a lysis reagent; Sodium oxide and hydroquinone; binding enhancers including isopropanol and sodium chloride. The invention can integrate nucleic acid extraction and amplification detection in a fully enclosed environment, which is efficient, convenient and accurate.

Description

technical field [0001] The present invention relates to a kit for detecting methylation of PAX1 gene in cervical cancer. Background technique [0002] The nucleic acid diagnostic analysis method based on PCR technology is the core technology in molecular diagnosis. Integrating the automatic nucleic acid extraction process and the PCR amplification detection process to build an integrated nucleic acid extraction and amplification detection system has important practical significance for further improving the efficiency of nucleic acid diagnosis and analysis and realizing the automation of the entire nucleic acid diagnosis and analysis process. [0003] Chinese patent application 201610266982.8 discloses a detection kit for cervical cancer cells based on boron-doped graphene quantum dots, which belongs to the field of biological detection. The cervical cancer cell detection kit includes reagents A, B and C, reagent A is a boron-doped graphene quantum dot solution, reagent B i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12M1/34C12M1/00
CPCC12Q1/6886C12Q2600/154
Inventor 刘淑园陈华云张天海王盼盼
Owner GUANGZHOU HEAS BIOTECH CO LTD