Quadruple probiotic solid beverage and preparation method thereof
A technology of quadruple probiotics and solid beverages, applied to bacteria, lactobacillus, bifidobacteria and other directions used in food preparation, can solve the problems of uneven product quality and effect, ensure the number of viable bacteria, reduce harmful Bacteria, the effect of enhancing immunity
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[0051] Example 1: A quadruple probiotic solid beverage and its preparation method
[0052] 1. Formula
[0053] Strictly screened and commercially available raw materials that meet the corresponding national standards / industry standards and national regulations are qualified to obtain various raw materials. Weigh 8kg of Lactobacillus rhamnosus lyophilized powder, 5kg of Lactobacillus reuteri lyophilized powder, 5kg of Bifidobacterium animalis lyophilized powder, 5kg of Bifidobacterium lactis lyophilized powder, 8.5kg of stachyose, and 31kg of glucose. , 24kg of whole milk powder, 3.5kg of isomalt oligosaccharide, 2kg of xylitol, 2kg of resistant dextrin, 5.906kg of banana powder, vitamin B 1 0.0015kg, vitamin B 2 0.001kg, vitamin B 6 0.001kg and 0.0905kg of zinc gluconate.
[0054] 2. Preparation method
[0055] 1) First add vitamin B 1 0.0015kg, vitamin B 2 0.001kg, vitamin B 6 Mix 0.001kg with 0.0905kg of zinc gluconate, then mix with 2kg of glucose, 8.5kg of stachyose, 3.5kg o...
Example Embodiment
[0057] Example two
[0058] 1. Sensory evaluation
[0059] Take 6 grams of the product prepared in Example 1 and add it to 30-40 ml of warm water (below 40° C.) to completely dissolve. Ten sensory assessors were selected, and after observing and tasting, the color, state, smell and smell of the product of the present invention were comprehensively evaluated according to the assessment method in Table 1, and the average score was taken as the comprehensive score.
[0060] Table 1 Sensory evaluation methods and results
[0061]
[0062] The sensory evaluation conclusions are drawn from Table 1: The product of the present invention is white to light yellow in color, uniform, powdery, without agglomeration, no external impurities visible to the naked eye, and has a delicate and sweet taste, pleasant taste and no peculiar smell.
[0063] 2. Immunity comparison experiment
[0064] Experimental animals: Kunming mice aged 4 to 5 weeks, 18 to 22 g, male, 10 in each group, 90 in total.
[0065] Ex...
Example Embodiment
[0085] Example three
[0086] Screening of Lactobacillus reuteri freeze-dried protective agent:
[0087] The protective agent generally has more than three hydrogen bonds or some ionic groups, which can replace water and bind to the protein of the bacteria during the protection process, thereby reducing the formation of ice crystals and protecting the bacteria from damage. The sterilization conditions of the protective agent are the temperature of 115°C and the sterilization time of 30 minutes.
[0088] As a protective agent, skimmed milk powder can form a protective layer on the cell surface to protect the cell wall from damage. So choose skimmed milk powder as the basic protective agent. Glucose and sucrose were added to the basic protective agent at the ratios of 2%, 4%, 6%, 8%, and 10%, respectively, to investigate the effect of adding different concentrations of glucose and sucrose on the protective effect of Lactobacillus reuteri.
[0089] Table 4 Effects of glucose and sucros...
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