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A method for prolonging the shelf life of liquid plant lactobacillus products for fish

A plant lactobacillus and shelf life technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of inconvenient transportation of packaging containers, cumbersome fixing methods, and expensive products, and ensure the survival of bacterial liquid. The number of bacteria, beneficial to storage and transportation, and easy to implement

Active Publication Date: 2020-04-10
GUANGXI ACADEMY OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to promote the application of plant Lactobacillus, experts and scholars have done a lot of research on immobilization technology. Although the immobilization technology solves the shortcomings of large packaging containers and inconvenient transportation, the immobilization method is cumbersome, has high requirements for instruments and equipment, and has low cell viability. Weak and easily polluted, the product is expensive and the cost of use is high

Method used

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  • A method for prolonging the shelf life of liquid plant lactobacillus products for fish
  • A method for prolonging the shelf life of liquid plant lactobacillus products for fish
  • A method for prolonging the shelf life of liquid plant lactobacillus products for fish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Preparation of Lactobacillus plantarum for fishery

[0040] Bacterial source: obtained by the Guangxi Academy of Fishery Sciences from the high-yield shrimp pond bottom mud by enrichment, separation and culture, and identified as Lactobacillus plantarum.

[0041] Fermentation medium: molasses 30.0g, CH 3 COONa 6.0g, NaCl 6.0g, KH 2 PO 4 2.5g, K 2 HPO 4 2.0g, MgSO 4 ·7H 2 O 0.60g, yeast extract 1.5g, peptone 2.0g, CaCl 2 50.0mg, MnSO 4 25.0mg, FeSO 4 5.0mg, adjust pH value to 6.0, aerate tap water 1000ml.

[0042] Expanded culture: use 100L seed tank for fermentation and culture, insert the prepared seed bacterial liquid into the sterilized fermentation medium with an inoculation amount of 5% by volume, the loading coefficient is 90% (v / v), and stir evenly After that, the cells were incubated at 40°C for 48 hours in a constant temperature and airtight manner, and the culture was completed by placing the tank.

[0043] 2 batches of 1m were carried out accord...

Embodiment 2

[0048] Effect of initial pH value of bacterial solution on preservation effect of liquid Lactobacillus plantarum for fishing

[0049] The Lactobacillus plantarum bacteria liquid of batch No. 1 prepared in Example 1 was adjusted with 2% dipotassium hydrogen phosphate buffer to adjust the initial pH value of the bacterial liquid, and 5 gradients were adjusted in total, which were 4.5, 5.0, 5.5, 6.0, 6.5, Each gradient of 7.0 and 7.5 was set to 2 repetitions, and then divided into 2L polyethylene plastic bottles, a total of 14 bottles, placed in indoor natural conditions for 6 months and 12 months, and then sampled to determine the bacterial concentration of the bacterial liquid And calculate the survival rate, the test results are shown in Table 2.

[0050] Table 2 The survival rate of Lactobacillus plantarum for fishery at different pH values ​​at 6 months and 12 months

[0051]

[0052]

[0053] From the measurement data in Table 2, it can be seen that Lactobacillus pla...

Embodiment 3

[0055] The effect of vitamin C on the preservation of liquid Lactobacillus plantarum for fishing

[0056] The Lactobacillus plantarum bacteria liquid of batch No. 2 prepared in Example 1 was added with vitamin C of 0.0g / L, 0.25g / L, 0.5g / L, 0.75g / L and 1.0g / L in the bacterial liquid, and the After adjusting the pH of the bacterial solution to 6.0 with 2% dipotassium hydrogen phosphate buffer, it was then dispensed into 2L polyethylene plastic bottles, with 2 replicates for each gradient, a total of 12 bottles, and stored in indoor natural conditions for 6 months After 12 months, samples were taken to measure the bacterial concentration of the bacterial liquid and the survival rate was calculated. The test results are shown in Table 3.

[0057] Table 3 The bacterial survival rate of Lactobacillus plantarum for fishery under different vitamin C concentrations for 6 months and 12 months

[0058]

[0059] The results from Table 3 show that adding 0.25 to 1.0 g / L vitamin C to th...

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Abstract

Relating to the technical field of fishery microbial preparations, the invention provides a method for extending the shelf life of a fishery liquid lactobacillus plantarum product. The method includes: adding vitamin C into a fishery lactobacillus plantarum bacterial liquid to obtain a bacterial liquid containing 0.25-1.0g / L vitamin C; using a phosphate buffer solution to adjust the pH value of the obtained bacterial liquid to 4.5-7.0; sealing and packing the well adjusted lactobacillus plantarum, and conducting preservation at room temperature. The method provided by the invention has the advantages of simple operation, low cost, easy implementation and simple requirements for preservation conditions, can prolong the shelf life of the fishery liquid lactobacillus plantarum product and ensure the bacterial concentration, the survival rate of bacterial liquid cells preserved for 6 months can reach 89.36%, and the survival rate of bacterial liquid cells preserved for 12 months can reach80.85%, therefore the fishery liquid lactobacillus plantarum product preservation method provided by the invention is worthy of vigorous popularization and application.

Description

technical field [0001] The invention belongs to the technical field of fishery microbial preparations, and in particular relates to a method for prolonging the shelf life of fishery liquid Lactobacillus plantarum products. Background technique [0002] Lactic acid bacteria (LAB) is a general term for a class of bacteria that can utilize fermentable carbohydrates to produce large amounts of lactic acid. Such bacteria are widely distributed in nature and have rich species diversity. Due to the irrational use of antibiotics and hormone drugs in aquatic products, the problem of pesticide residues has caused a huge threat to human health. In the field of aquaculture, people are increasingly advocating ecological aquaculture technology. In the United States, nearly 30 of the more than 40 kinds of feed microorganisms specified by the FDA (1989) are lactic acid bacteria. The microbial species approved by the Ministry of Agriculture of my country in 1994 include Bacillus cereus, B...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/04C12R1/25
CPCC12N1/04C12N1/20
Inventor 黎建斌李大列黄黎明杨学明吕敏陈福艳罗帮
Owner GUANGXI ACADEMY OF FISHERY SCI
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