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A peptide and gold nanobody targeting HIV envelope protein gp120

An envelope protein and gold nanotechnology, applied in the field of nanomedicine, can solve problems such as adverse side effects, reduced antiviral treatment effect, and inability to eradicate

Active Publication Date: 2021-10-12
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] HIV is easy to induce drug-resistant mutations, and has great toxic and side effects on the human body. At the same time, there are problems such as rapid drug failure, high cost, and inability to eradicate
With the development of modern technology and medicine, although highly effective antiretroviral therapy (HAART) and the technology of predicting whether a patient is infected with HIV have been improved, the impact of HIV virus on the human body cannot be eradicated
[0003] At present, reverse transcriptase inhibitors, protease inhibitors and integrase inhibitors are the most used, but long-term medication will cause drug resistance and adverse side effects in the human body, which will also reduce the effect of antiviral therapy

Method used

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  • A peptide and gold nanobody targeting HIV envelope protein gp120
  • A peptide and gold nanobody targeting HIV envelope protein gp120
  • A peptide and gold nanobody targeting HIV envelope protein gp120

Examples

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Embodiment 1

[0023] Example 1: A gold nanobody targeting HIV envelope protein gp120

[0024] 1. Design of peptide fragments

[0025] Based on the structure of gp120 natural antibody, a polypeptide sequence CD5 was designed: Cys-Ala-Lys-Trp-Arg-Pro-Leu-Arg-Tyr-Ser-Asp-Tyr-Pro-Ser-Asn-Ser-Asp-Tyr- Tyr-Asp-Cys.

[0026] It can be fixed on the surface of gold nanoparticles through the sulfhydryl groups of cysteines at both ends, thereby forming a specific ring conformation. The peptide was synthesized by Jill Biochemical Co., Ltd.

[0027] 2. Preparation of anti-HIV gold nanobody

[0028] Based on the construction method of artificial antibodies in our laboratory, see Yan G H, Wang K, Shao Z, et al. Artificial antibody created by conformational reconstruction of the complementary-determining region on gold nanoparticles: Proceedings of the National Academy of Sciences of the United States of America, 2018, 115(1): E34. Gold nanoparticles are selected as the carrier, the synthesized polype...

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Abstract

The invention relates to a polypeptide and a gold nanobody capable of targeting HIV envelope protein gp120. The artificial antibody is composed of gold nanoparticles and a designed polypeptide sequence. The prepared gold nanobody can enhance the binding strength of the drug to the target, that is, when the concentration of virus gp120 in the body is very low, it can still maintain a high binding strength with it. At the same time, gold nanoantibodies have the characteristics of small particle size, excellent dispersion, and good stability. They can reach places that ordinary drugs cannot reach, and can bind to viruses more strongly. Therefore, this artificial antibody has broad application prospects in the field of biomedicine. .

Description

technical field [0001] The invention belongs to the field of nanomedicine, and specifically relates to a polypeptide and a gold nanobody capable of targeting HIV envelope protein gp120. Background technique [0002] HIV is easy to induce drug-resistant mutations and has serious side effects on the human body. At the same time, there are problems such as rapid drug failure, high cost, and inability to eradicate. With the development of modern technology and medicine, although highly effective antiretroviral therapy (HAART) and the technology of predicting whether a patient is infected with HIV have been improved, the impact of HIV virus on the human body cannot be eradicated. [0003] At present, reverse transcriptase inhibitors, protease inhibitors and integrase inhibitors are the most used, but long-term medication will cause drug resistance and adverse side effects in the human body, which will also reduce the effect of antiviral therapy. Therefore, it is imminent to deve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/10A61K39/44A61K47/52A61P31/18
CPCA61K39/44A61K47/52A61P31/18C07K16/1063
Inventor 曹傲能倪睿夏渝江王海芳
Owner SHANGHAI UNIV
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