A kind of preparation method of virus stock solution

Abstract

The invention relates to a preparation method of a virus stock solution. The host cells are cleaned with a serum-free medium; dextran, phosphate buffer and virus are prepared into a mixed solution; the mixed solution is added to the treated host cells Carry out adsorption infection; culture the treated host cells with a medium containing fetal bovine serum; freeze and thaw the treated host cells to release the virus, and then centrifuge to obtain the virus stock solution. The present invention replaces polybrene by adopting dextran, because dextran itself is a kind of cationic polymer, it is combined with the negatively charged virus and is close to the cell membrane to be taken up, and has no toxic effect on the cell itself, fundamentally In fact, it can improve the growth rate and proliferation efficiency of host cells, and play a significant role in promoting the amplification of viruses, so that the virus titer of the prepared virus stock solution is high.

Description

technical field

[0001] The invention relates to a preparation method of virus stock solution. Background technique

[0002] The virus stock solution can be used to prepare vaccines, and can also be added as an indicator virus in the virus inactivation verification experiment. When used as an indicator virus in the virus inactivation verification experiment, the greater the virus titer of the virus stock solution, the more virus that needs to be added. Fewer stock solutions can reduce the cost of virus inactivation verification experiments.

[0003] In the prior art, the stock solution of heterotropic murine leukemia virus is usually inoculated into host cells with a solution containing heterotropic murine leukemia virus, and co-cultivated. Polybrene needs to be added to the culture medium to improve heterotropic The infection efficiency of mouse leukemia virus entering host cells (the main function of polybrene is to improve the efficiency of retrovirus infection of cells, ...