Lateral-flow test paper detecting device and manufacturing method thereof

Abstract

The invention discloses a lateral-flow test paper detecting device and a manufacturing method thereof. The lateral-flow test paper detecting device comprises lateral-flow test paper (1) and an alternating-current dielectrophoresis chip (2), wherein a detecting pad (4) comprises a detecting line (7) and a contrast line (8); a capturing probe is fixedly arranged on the detecting line (7), and a contrast probe is fixedly arranged on the contrast line (8); the alternating-current dielectrophoresis chip (2) for controlling distribution of the capturing probe comprises a glass plate (9), a first micro electrode (10) and a second micro electrode (12), wherein a through hole (11) for dropwise adding a capturing probe solution and / or a contrast probe solution to the surface of the detecting pad (4)is formed in the glass plate (9); the first micro electrode (10) is fixedly arranged on the glass plate (9) and is positioned at one side of the through hole (11); the second micro electrode (12) isfixedly arranged on the glass plate (9) and is positioned at the other side of the through hole (11); and the first micro electrode (10) and the second micro electrode (12) which are connected with apower supply generate positive dielectrophoretic motion electric field force, so that the capturing probe on the whole thickness is gathered in a visible area at the upper layer of the detecting pad.

Description

technical field [0001] The invention relates to the technical field of instant biological test paper detection, in particular to a lateral flow test paper detection device and a preparation method thereof. Background technique [0002] As a paper-based real-time detection and diagnostic device with great potential, lateral flow test paper is widely used in global public health care, agricultural testing, food safety and environmental monitoring and other fields (patent US201662425839P). It has attracted worldwide attention, especially in remote and underdeveloped areas, because of its advantages of easy portability, low cost, and random disposal. Generally, lateral flow test paper consists of four parts: immersion pad, detection pad, absorbent pad and adhesive backing sheet. Wherein, the side of the immersion pad close to the detection pad is loaded with dissolvable chromogenic particles, and the detection line and control line on the detection pad are respectively loaded w...

AI Technical Summary

Problems solved by technology

However, increasing the concentration of capture probes without limit will not continuously increase the detection signal, but will cause waste of capture probes and increase the manufacturing cost of lateral flow test strips

This is because the chromogenic particles loaded on the immersion pad are limited, and increasing the concentration of the capture probe can only increase the capture probability of the chromogenic particles to a certain extent, and the chromogenic particles that are finally captured on the detection line are limited; in addition, when the detection After the capture probe on the surface of the fiber on the line reaches saturation, the excess capture probe cannot be fixed on the detection line and will be washed away with the liquid, which cannot effectively capture the chromogenic particles

Images