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A kind of extraction method of artificial forest bark saponins active components

An active ingredient and extraction method technology, which is applied in the field of extraction of plantation bark saponins active ingredients, can solve the problems of low utilization rate and low extraction rate of active substances, and achieves promoting pulverization and emulsification, improving extraction rate and yield. high effect

Active Publication Date: 2021-06-18
NANJING FORESTRY UNIV
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Problems solved by technology

[0005] Aiming at the problems of low bark utilization rate and low extraction rate of active substances in my country's plantation forest non-wood resources, the present invention proposes a method for extracting saponin active components from plantation forest bark, which is simple in operation, good in feasibility, and can significantly Improve the extraction efficiency of saponin active substances

Method used

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  • A kind of extraction method of artificial forest bark saponins active components
  • A kind of extraction method of artificial forest bark saponins active components
  • A kind of extraction method of artificial forest bark saponins active components

Examples

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Effect test

Embodiment 1

[0028] Extraction of Total Saponins from Black Wattle Bark

[0029] Take 3 parts of 3g (accurate to 0.001) black wattle bark powder raw material in 100mL Erlenmeyer flask, add 0.5mg / mL cellulose complex enzyme 50% ethanol solution at a ratio of 1:10, and ultrasonically treat at 50°C for 40min (ultrasonic frequency 40KHz, ultrasonic power 120W), filter, transfer the filtrate to a 100mL volumetric flask, and set the volume to 100mL.

Embodiment 2

[0037] Extraction of Total Saponins from the Bark of Acacia

[0038] Take 3 parts of 3g (accurate to 0.001) bark powder raw material of Acacia acacia respectively in 100mL Erlenmeyer flask, add 0.5mg / mL cellulose compound enzyme 50% ethanol solution with the ratio of material to liquid 1:10, at 50 ℃ Sonicate for 40 minutes (ultrasonic frequency 40KHz, ultrasonic power 140W), filter, transfer the filtrate to a 100mL volumetric flask, and dilute to 100mL.

Embodiment 3

[0045] Extraction of Total Saponins from Yew Bark

[0046] Take 3 parts of 3g (accurate to 0.001) yew bark powder raw material in 100mL Erlenmeyer flask, add 0.5mg / mL cellulose complex enzyme 50% ethanol solution at a material-to-liquid ratio of 1:10, and ultrasonically treat at 50°C After 40 minutes (ultrasonic frequency 40KHz, ultrasonic power 160W), filter, transfer the filtrate to a 100mL volumetric flask, and dilute to 100mL.

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Abstract

The invention belongs to the technical field of plant extraction, and discloses a method for extracting saponin active ingredients from artificial forest bark, comprising the following steps: 1) dissolving cellulose compound enzyme in aqueous ethanol solution to obtain a cellulose compound enzyme solution; 2) dissolving cellulose compound enzyme in an aqueous ethanol solution; Plantation bark powder and cellulose compound enzyme solution are mixed according to the ratio of material to liquid mass ratio of 1:6 to 12 to obtain a mixed material liquid; 3) the mixed material liquid is placed at 30 to 70 °C, and ultrasonic enzymolysis is performed for 30 to 30 °C. After 80 minutes, solid-liquid separation was carried out, and the liquid phase components were collected as saponin active components. The enzyme treatment and ultrasonic-assisted coupling extraction of the present invention have the highest yield of active ingredients, which is more than 20% higher than the traditional organic solvent extraction method and more than 10% higher than the ultrasonic extraction method. It is a low-temperature, green and efficient extraction method .

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a method for extracting saponin active components from plantation bark. Background technique [0002] Artificial forests are forests cultivated by means of artificial sowing, planting or cuttings and other technical measures, and are established by artificial afforestation or artificial reforestation [the definition of "artificial forest" in the forest inventory work of the State Forestry Administration]. Artificial forests can be the introduction of new species or enhanced management of native tree species. According to tree species, they can be divided into artificial masson pine forests, artificial fir forests, and artificial eucalyptus forests. Compared with natural forests, artificial forests have the characteristics of fast growth, high growth volume, high profits, easy processing and utilization, and ecological benefits. [0003] my country is the cou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/48A61K36/13A61K36/61A61K129/00
CPCA61K36/13A61K36/48A61K36/61A61K2236/19A61K2236/333A61K2236/53
Inventor 李迅周菲菲张旭群林叶指王飞姜萍
Owner NANJING FORESTRY UNIV
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