Nucleic acid extraction method and device

Abstract

The invention discloses a nucleic acid extraction method and device. The device includes a sample extraction module used for holding a nucleic acid sample and extracting nucleic acid from the sample,a magnetic bead injection pump used for injecting a solution mixing micron magnetic beads into the sample extraction module, a magnetic field generation module used for generating high intensity magnetic fields, a sample cracking module used for separating the nucleic acid from the sample, and an eluent injection pump used for injecting an eluent into the sample extraction module to separate the nucleic acid from the beads so that the nucleic acid can be obtained by recycling the beads. The method and device for effectively, rapidly and semi-automatically extracting the nucleic acid in biological samples with large volume are provided; through the method, rapid extraction of the nucleic acid in samples of up to 10 ml can be realized, and the extraction time is shorter than traditional ways; and the whole extraction link can be operated automatically and combined with other detection devices later, and the purpose of fully automated detection can be achieved.

Description

technical field [0001] The present invention relates to the field of continuous fluid separation. More specifically, it relates to a nucleic acid extraction method and device. Background technique [0002] Molecular diagnosis uses nucleic acid as the detection object and is mainly used in various biological diagnoses. Before the sample is subjected to molecular diagnosis, it is first necessary to extract nucleic acid from the sample to be tested. The concentration and purity of nucleic acid directly affect the accuracy of molecular diagnosis. Traditional nucleic acid extraction methods generally include density gradient centrifugation, phenol / chloroform, phenol, and isoamyl alcohol extraction, etc., but these methods are either toxic, time-consuming, or require large and complex devices, which restrict their methods. In recent years, the magnetic bead method has been more and more widely used in the extraction of nucleic acids in samples. The general steps of this method ...

AI Technical Summary

Problems solved by technology

[0003] However, the conventional magnetic bead method for nucleic acid extraction has the following disadvantages: First, the sample volume for nucleic acid extraction by the magnetic bead method is limited, generally ranging from tens of microliters to hundreds of microliters

Secondly, the operation steps of manual magnetic bead extraction of nucleic acid are relatively complicated and require experienced technicians to perform skilled operations; while the common automated nucleic acid extraction devices on the market are large in size, relatively expensive, and not easy to carry, which is difficult to meet on-site testing needs

Finally, the recently developed microfluidic magnetic bead extraction method for nucleic acid generally has a small volume of samples to be tested and a slow extraction speed.

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