Method for detecting single nucleotide polymorphism site of cattle ret gene, detection kit and application thereof

A single nucleotide polymorphism, detection kit technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problem of offspring sickness, inability to screen for HSCR pathogenic mutation sites, household heads or feedlots Losses and other issues, to achieve important effects of economic value
CN108841933BActive Publication Date: 2021-07-09XINYANG NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
XINYANG NORMAL UNIVERSITY
Publication Date
2021-07-09

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Abstract

The invention discloses cattle RET The detection method of gene single nucleotide polymorphism site comprises the following steps: to contain RET The whole genome DNA of the cattle to be tested is used as a template, and the primer pair P is used as a primer to amplify the cattle by PCR. RET Gene; with restriction endonuclease MaeI (XspI) Digest the PCR amplification product, and then perform non-denaturing polyacrylamide gel electrophoresis on the digested fragment; determine the yellow cattle according to the results of polyacrylamide gel electrophoresis combined with sequencing RET The genotype of the single nucleotide polymorphism site at position 34690 of the gene. The invention also discloses RET The application of the detection kit of single nucleotide polymorphism site in yellow cattle molecular breeding. because RET The gene has important biological functions. The single nucleotide polymorphism of the gene has a significant impact on the early body length, body height, and bust of cattle. It can be used for molecular marker-assisted selection of cattle and growth traits, and then quickly establish excellent genetic resources. cattle population.
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Description

technical field

[0001] The invention belongs to the technical field of biogenetic engineering, and in particular relates to a method for detecting a single nucleotide polymorphism site of a cattle RET gene, a detection kit and an application thereof. Background technique

[0002] Cattle are one of the main livestock resources in my country, but now they are facing the pressure of insufficient provenance of excellent cattle breeds and poor genetic quality of the population. Traditional breeding methods are based on determining the phenotype of animals and using the genetic information of their parents, ancestors and other relatives in animal models to achieve genetic evaluation. Generally, it is assumed that traits are affected by genetic differences of many genes, so each gene is considered There are relatively small contributions to the trait, with the result that the estimates of the trait are inevitably somewhat biased. Molecular genetic markers are one of the fastest-gr...

Claims

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