Early detection and diagnosis kit and device for Parkinson's disease

A diagnostic kit and technology for Parkinson's disease, applied in the field of biomedicine, can solve the problems of unable to meet the needs of rapid diagnosis, time-consuming and labor-intensive data analysis, unbearable for patients, etc. less traumatic effect

Active Publication Date: 2018-11-20
PEKING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the gene expression profile detection technology in blood has the following disadvantages: the above two studies screened all the genes expressed in the blood by the method of gene chips, which is expensive and unaffordable for patients clinically.
In addition, due to the large number of genes, data analysis is time-consuming and labor-intensive, which cannot meet the clinical needs for rapid diagnosis
Moreover, both studies used RNA extracted from whole blood cells (primarily white blood cells), which frequently undergo nuclear and transcriptional changes during disease states and are therefore susceptible to interference from other diseases

Method used

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  • Early detection and diagnosis kit and device for Parkinson's disease
  • Early detection and diagnosis kit and device for Parkinson's disease
  • Early detection and diagnosis kit and device for Parkinson's disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Screening experiment: In this embodiment, the specific technical methods used are as follows:

[0040] 1. Subjects

[0041] This study was approved by the ethical review boards of all participating institutions. Both the discovery cohort (total of 48: 12 healthy controls, 36 PD patients) and the validation cohort (total of 340: 135 healthy controls, 205 PD patients) were from Beijing Tiantan Hospital. All participants provided informed consent and underwent medical history taking, neurological examination, laboratory tests, and psychological evaluation by a neurologist. All control subjects were community volunteers with mental status examination scores >27, paragraph recall scores >6, no history of neurological disease, and no history or evidence of cognitive or functional decline. All PD patients met the clinical diagnostic criteria of the UKPD social brain bank. PD patients were further classified to approximate disease stage based on the UPDRS Part III state-moto...

Embodiment 2

[0064] In this embodiment, the components of the kit are as follows in Table 1:

[0065] Table 1

[0066] SuperScript TM III RT Reverse Transcriptase / Platinum TM Taq Enzyme Mix

2X mix buffer

Contains 0.2mM dNTPs, 3mM MgS0 4

CHCHD2 probe

200nM

CHCHD2 upstream and downstream primer pair

500nM each

AHSP probe

200nM

AHSP upstream and downstream primer pair

500nM each

droplet stabilizer

1x

h 2 o

[0067] Note: CHCHD2 / AHSP probes and primers are the same as those of digital PCR in Example 1.

[0068] Subsequently, 135 healthy controls and 205 PD patients were tested using the above kit components by digital PCR method, and the results were consistent with our results in the discovery cohort ( Figure 2A ). The ROC curve showed that the sensitivity and specificity of CHCHD2 in detecting PD exceeded 80%, suggesting that it has a very good diagnostic value. Compared with healthy con...

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Abstract

The invention discloses an early detection and diagnosis kit and device for Parkinson's disease. The kit comprises an agent for detecting the expression of CHCHD2 genes in red blood cells. With the technical solution, PD patients and a health control can be distinguished only by detecting the expression of CHCHD2 genes in red blood cells, and early diagnosis and early warning on the PD patients can be realized; the required sample is venous blood of which the extraction is clinically very routine, so the extraction is simple, the trauma is small, and the patients do not reject, the detection process is simple, the cost is low, the subsequent data analysis saves time and effort, results are fast to provide, and the clinical promotion is easy.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an early detection and diagnosis kit and device for Parkinson's disease. Background technique [0002] Parkinson's disease (PD) is a common clinical neurodegenerative disease. The current diagnosis of the disease is mainly based on medical history, observation of major motor indicators, and response to drug therapy. However, 50% of dopaminergic neurons are already apoptotic at the time of symptom onset, leading to delayed diagnosis, poor treatment response and poor prognosis. Therefore, it is urgent to develop a method capable of early detection and diagnosis of PD. [0003] At present, research on PD biomarkers mainly focuses on the discovery of protein biomarkers in cerebrospinal fluid, and α-synuclein and its variants have received the most attention. However, the main disadvantages of detecting PD protein biomarkers in cerebrospinal fluid are: 1) the collection of cereb...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883
CPCC12Q1/6883
Inventor 章京刘小旦王琪龙
Owner PEKING UNIV
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