High-efficiency interception apparatus for cell culture and interception method thereof
A cell culture and interception device technology, applied in the field of cell culture, can solve the problem of easy clogging of ATF intercepted cells, and achieve the effect of improving interception efficiency
Active Publication Date: 2018-11-23
石药集团明复乐药业(广州)有限公司
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Problems solved by technology
[0003] Aiming at the deficiencies of the prior art, the object of the present invention is to provide a downstream purification process that reduces the difficulty of the downstream purification process where the residual cells in the harvest supernatant are increased by the single use of the acoustic interception system, and solves the problem that the single use of ATF interception cells is easy to clog High-efficiency interception device and interception method thereof
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[0020] Below, in conjunction with accompanying drawing and specific embodiment, the present invention is described further:
[0021] Such as figure 1 As shown, a high-efficiency interception device for cell culture includes a reactor, an acoustic interceptor, a hollow fiber column and a harvest tank, and the reactor is connected to the inlet port of the external circulation pump and the acoustic interceptor through pipelines in sequence; The settling port of the acoustic interceptor is connected with the reactor, the outlet port of the acoustic interceptor is connected with the hollow fiber column through a pipeline, and a liquid outlet pump is installed at the pipeline between the outlet port and the hollow fiber column; the hollow The liquid outlet of the fiber column is connected to the harvesting pump and the harvesting tank sequentially through pipelines, and the hollow fiber column collects the trapped cells into a reactor connected to the hollow fiber column.
[0022] ...
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Abstract
The invention relates to a high-efficiency interception apparatus for cell culture. The high-efficiency interception apparatus comprises a reactor, an acoustic interception device, a hollow fiber column and a harvesting tank, wherein the reactor is successively connected with an inlet end of an outer circulating pump and an inlet end of the acoustic interception device by virtue of pipelines; a settling opening of the acoustic interception device is connected with the reactor, an outlet of the acoustic interception device is connected with the hollow fiber column by virtue of a pipeline, and the pipeline between the outlet and the hollow fiber column is provided with a liquid outlet pump; and the liquid outlet of the hollow fiber column is successively connected with a harvesting pump andthe harvesting tank by virtue of the pipeline, and cells intercepted by the hollow fiber column are collected into the reactor connected with the hollow fiber column. By adopting the high-efficiency interception apparatus, the interception efficiency can be greatly improved, and 90 percent or more of interception efficiency can be satisfied.
Description
technical field [0001] The invention relates to the field of cell culture, in particular to a high-efficiency retaining device for cell culture and a retaining method thereof. Background technique [0002] There are more and more varieties of genetic engineering drugs produced by animal cells, especially the emergence of engineered antibodies, and the output has become the bottleneck of production. The use of perfusion culture systems to replace traditional batch production processes has attracted increasing attention. The use of a perfusion culture system can greatly increase cell density and yield, and greatly reduce the investment in equipment and delivery rooms. In order to carry out effective perfusion culture, the core is to have a high-efficiency, low-cost, and long-term use of a perfusion system that traps cells in the reaction tank. So far, interception devices include acoustic wave interception, ATF interception, etc., but each method has its own shortcomings. Fo...
Claims
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IPC IPC(8): C12M3/00C12M1/42
CPCC12M33/14C12M35/04
Inventor 王森隆杰锋毛伟斯区则凡郭俊钰李添
Owner 石药集团明复乐药业(广州)有限公司