Building method of gastroesophageal reflux disease animal model

A gastroesophageal reflux and animal model technology, applied in medical science, veterinary instruments, veterinary surgery, etc., can solve the problems of destroying the lower esophageal sphincter and gastric physiological tissue structure, and cannot fully explain the incidence of reflux esophagitis

Inactive Publication Date: 2018-11-27
广西中医药大学第一附属医院
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, the generally recognized methods for establishing animal models of lower esophageal sphincter relaxation and delayed gastric emptying are mainly cardiac sphincterotomy, partial cardiac sphincterectomy and pyloric ligatio

Method used

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Embodiment 1

[0016] A method for establishing an animal model of gastroesophageal reflux disease, comprising the following steps:

[0017] Preparation of experimental animals and experimental equipment:

[0018] Experimental animals: 45 SD rats of healthy and clean grade, male or female, weighing 200-220g, were kept in a clean-grade animal room with a 12-h rhythm, room temperature (22±2)°C, humidity 60%-70%, and rats were fed with whole All mice were fed with cheap pellet feed and had free access to drinking water; all experimental animals were provided by the Experimental Animal Center of Guangxi Medical University. License number: SYXK (Beijing) 2013-0012, certificate number: 11401500004829.

[0019] Main experimental equipment:

[0020] Iodoacetamide: Siogma, lot number: 1001437587. Sucrose: Bairdy Corporation, lot number: 0552C003. Pediatric 8Fr double-lumen catheter (Bad Forley Catheter): Bard Company of the United States, registration standard number: YZB / MAL0196-2013. The high-...

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Abstract

The invention discloses a building method of a gastroesophageal reflux disease animal model. The building method comprises the following steps that S1, 10% chloral hydrate (3 ml/kg) is injected into the abdomen of an SD mouse, and after being anesthetized, the mouse lies on the back, and is fixed to a wooden plate, and subjected to conventional skin preserving and disinfection; S2, the maxilla ofthe SD mouse is strutted and fixed, a pediatric 8Fr dual-cavity catheter is inserted via the mouth for about 15 cm, the median incision below the xiphoid is taken and is 3 cm in length, the abdomen isdissected layer by layer, the catheter enters the abdomen, the junction of the lower esophagus and the stomach esophagus is fully exposed and dissociated, two rubber bands are placed in the rear portion of the esophagus, and the near end and the far end of the lower esophageal sphincter are labeled. The building method has the advantages that the gastroesophageal reflux disease animal model whichis manufactured by superimposing balloon dilatation of the pediatric 8Fr dual-cavity catheter and the iodoacetamide gavage method has the digestion power obstacle feature, and typical clinical indexes and pathological features, and a normal physiological anatomical structure of an animal can also be maintained.

Description

technical field [0001] The invention relates to a method for establishing an animal model of gastroesophageal reflux disease. Background technique [0002] Gastroesophageal reflux disease (GERD) refers to a group of diseases in which gastric contents reflux into the esophagus, causing discomfort symptoms and complications, including two types of syndromes, intraesophageal and extraesophageal. The pathogenesis of GERD is not well understood, but motility abnormalities play a causative role in reflux disease. Abnormal motility refers to digestive system motility disorders. Among the many causes of digestive system motility disorders, lower esophageal sphincter relaxation and delayed gastric emptying are considered to be the main causes of gastroesophageal reflux. The treatment of the etiology of GERD has always been a focus of attention and research by scholars at home and abroad. Appropriate animal models of GERD are of great value for the exploration of the pathogenesis of ...

Claims

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Application Information

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IPC IPC(8): A61D1/00
CPCA61D1/00
Inventor 谢胜刘礼剑刘园园刘倩黄晓燕杨成宁陈广文李建锋谢洁如李娟
Owner 广西中医药大学第一附属医院
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