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Microbiological treating agent for degrading aflatoxin B1, preparation method of treating agent and application

A technology of microbial treatment and aflatoxin, applied in the field of animal breeding, can solve the problems of high cost, incomplete detoxification, and destruction of nutrition, and achieve the effects of simple operation, inhibition of toxin production, and low cost

Active Publication Date: 2018-11-27
LUZHOU ZHENGTAI BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the present invention provides a microbial treatment agent for degrading aflatoxin B1 and its preparation method and application, aiming at the problems of aflatoxin B1 detoxification method destroying nutrition, incomplete detoxification and high cost

Method used

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  • Microbiological treating agent for degrading aflatoxin B1, preparation method of treating agent and application
  • Microbiological treating agent for degrading aflatoxin B1, preparation method of treating agent and application
  • Microbiological treating agent for degrading aflatoxin B1, preparation method of treating agent and application

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preparation example Construction

[0039] The invention also discloses a preparation method of a microbial treatment agent for degrading aflatoxin B1, comprising the following steps:

[0040] Step 1. Preparation of probiotic complex:

[0041] Step 1.1, preparation of Saccharomyces cerevisiae culture solution: Inoculate Saccharomyces cerevisiae in Saccharomyces cerevisiae fermentation medium at an inoculum size of 4%-8% (V / V), at 25-30°C, 155-165r / min shaker culture, Harvest when cultured for 27 hours, the content of regulator bacteria is 1×10 9 CFU / ml;

[0042] Among them, the composition of Saccharomyces cerevisiae fermentation medium: glucose 20g, yeast powder 10g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 1g, distilled water 1.0L, natural pH;

[0043] Step 1.2, the preparation of Acetobacter pasteurian culture fluid: inoculate Acetobacter pasteurii in the acetic acid bacteria fermentation medium by the inoculum size of 4%-8% (V / V), at 25-35 ℃, 95-105r / min Cultivate on a shaking table, harvest...

Embodiment 1

[0066] A preparation method of a microbial treatment agent for degrading aflatoxin B1, comprising the following steps:

[0067] Step 1. Preparation of probiotic complex:

[0068] Step 1.1, preparation of Saccharomyces cerevisiae culture solution: Inoculate Saccharomyces cerevisiae in Saccharomyces cerevisiae fermentation medium with an inoculum size of 6% (V / V), cultivate at 29°C, 160r / min shaker, harvest when to be cultivated for 27h, adjust The content of bacteria is 1×10 9 CFU / ml;

[0069] Among them, the composition of Saccharomyces cerevisiae fermentation medium: glucose 20g, yeast powder 10g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 1g, distilled water 1.0L, natural pH;

[0070] Step 1.2, preparation of Acetobacter pasteurian culture fluid: inoculate Acetobacter pasteurianum in the acetic acid bacteria fermentation medium by the inoculum size of 6% (V / V), at 30 ℃, 100r / min shaker culture, to be cultivated for 36h When harvested, the content of regulator ...

Embodiment 2

[0084] A preparation method of a microbial treatment agent for degrading aflatoxin B1, comprising the following steps:

[0085] Step 1. Preparation of probiotic complex:

[0086] Step 1.1, preparation of Saccharomyces cerevisiae culture solution: Inoculate Saccharomyces cerevisiae in Saccharomyces cerevisiae fermentation medium with an inoculum size of 4% (V / V), cultivate at 30°C on a shaker at 155r / min, harvest when to be cultivated for 27h, adjust The content of bacteria is 1×10 9 CFU / ml;

[0087] Among them, the composition of Saccharomyces cerevisiae fermentation medium: glucose 20g, yeast powder 10g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 1g, distilled water 1.0L, natural pH;

[0088] Step 1.2, preparation of Acetobacter pasteurian culture fluid: Inoculate Acetobacter pasteurianus in the acetic acid bacteria fermentation medium by the inoculum size of 4% (V / V), at 35 DEG C, 95r / min shaker culture, to be cultivated for 36h When harvested, the content of ...

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Abstract

The invention discloses a microbiological treating agent for degrading aflatoxin B1, a preparation method of the treating agent and an application. The microbiological treating agent comprises a probiotic compound and coarse degrading enzyme of the aflatoxin B, wherein the volume ratio of the probiotic compound to the coarse degrading enzyme of the aflatoxin B is 1:3-3:1. The preparation method includes the steps: preparing the probiotic compound; preparing the coarse degrading enzyme of the aflatoxin B; mixing the probiotic compound and the coarse degrading enzyme of the aflatoxin B accordingto the volume ratio of 1:3-3:1 to prepare the microbiological treating agent for degrading the aflatoxin B1. The microbiological treating agent can relieve bad influence of the AFB1 (aflatoxin B1) onproduction performances of chickens and pigs.

Description

technical field [0001] The invention belongs to the technical field of animal feeding and breeding, and in particular relates to a microbial treatment agent for degrading aflatoxin B1, a preparation method and application thereof. Background technique [0002] Aflatoxin B1 (Aflatoxin B1, abbreviated as AFB1) is a derivative of dihydrofuran oxinone, which contains a difuran ring and an oxinone (coumarin). In the natural food of humans and animals, AFT (Aflatoxin) exists widely, and AFB1 is the most common and most harmful. AFB1 has strong toxicity to humans and some animals. For example, the hazards to poultry mainly include: Reduced feed intake, weight gain and feed conversion rate, infertility, abortion, pulmonary edema, increased susceptibility to diseases, etc.; the main hazards to pigs are: reduced feed intake, weight gain and feed conversion rate, infertility and abortion, Pulmonary edema, increased susceptibility to blight, etc.; hazards to dairy cows mainly include: ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C12N9/00A23L5/20C12R1/865C12R1/10C12R1/02C12R1/685C12R1/845
CPCA23L5/25A23L5/28C12N1/18C12N1/20C12N9/00
Inventor 张兴良夏锡兰钟秀
Owner LUZHOU ZHENGTAI BIOLOGICAL ENG CO LTD
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