Normal-temperature excrement sample preserving fluid

Abstract

The invention discloses a normal-temperature excrement sample preserving fluid. The preserving fluid comprises thiocyanate, organic alcohol, a complexing agent, phosphate buffer, Tween and serum albumin, wherein the concentration of the thiocyanate in the preserving fluid is 0.01-5M, the organic alcohol in the preserving fluid accounts for 30-60% of the weight of the preserving fluid; the concentration of the complexing agent in the preserving fluid is 0.001-0.35M; the phosphate buffer in the preserving fluid accounts for 5-15% of the preserving fluid; the Tween in the preserving fluid accounts for 0.05-1% of the weight of the preserving fluid; and the concentration of the serum albumin in the preserving fluid is 2.5-100mM. According to the normal-temperature excrement sample preserving fluid disclosed by the invention, the intestinal flora in excrement samples can be preserved at the normal temperature, the structure and diversity of the intestinal flora can be effectively maintainedwithin 14 days under room temperature conditions, the required conditions and cost for preservation of the intestinal flora can be reduced, and the intestinal flora in the excrements can be effectively preserved under special conditions such as outdoor sampling, sample transporting and the like.

Description

technical field [0001] The invention relates to a biological sample preservation solution, in particular to a stool sample preservation solution at room temperature. Background technique [0002] In recent years, studies on intestinal flora have been emerging, and the important role of intestinal flora on human health has attracted more and more attention. Intestinal flora is the normal microflora of the human intestinal tract, which constitutes a complex micro-ecological system with a wide variety and an astonishing number of characteristics. Studies have found that there are more than 800 types of microbiota residing in the intestinal tract. It is estimated that in a normal adult, the total weight of intestinal bacteria can reach 1-1.5kg. Intestinal flora plays an important role in the digestion process of the human body. It can participate in the synthesis and metabolism of vitamins, proteins, fats and other nutrients in the body. It has an important effect on the physi...

AI Technical Summary

Problems solved by technology

[0006] At present, the conventional method of feces preservation in hospitals is ultra-low temperature preservation at -80°C. This preservation method has the disadvantages of high requirements for equipment and high cost.

In addition, in some scenarios such as out sampling and sample transportation, it is impossible to achieve ultra-low temperature storage at -80°C. Especially during transportation, long-term storage at room temperature can easily lead to large changes in the composition of the community structure, which will affect the follow-up. The detection of the group will cause a large error

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