Extraction method and application of coriolus versicolor polysaccharide composition with high sugar content

A technology of Yunzhi polysaccharide and extraction method, which is applied in the directions of medical preparations, drug combinations, and pharmaceutical formulations containing active ingredients, can solve the problems of low sugar content of Yunzhi polysaccharide composition and lack of Yunzhi pretreatment, etc. The effect of increasing the content

Inactive Publication Date: 2018-11-30
陈德兴
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current water extraction method usually uses ethanol first and then heats and boils Yunzhi polysaccharides to extract polysaccharides from Yunzhi. It lacks the pre-treatment of Yunzhi, and the sugar content of the extracted polysaccharides from Yunzhi is low.

Method used

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  • Extraction method and application of coriolus versicolor polysaccharide composition with high sugar content
  • Extraction method and application of coriolus versicolor polysaccharide composition with high sugar content
  • Extraction method and application of coriolus versicolor polysaccharide composition with high sugar content

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The extraction method of polysaccharide polysaccharide composition with high sugar content comprises the following steps:

[0022] Step 1. After mixing Yunzhi and dry ice in a weight ratio of 1.5:1, pulverize it to below 120 mesh with a pulverizer to obtain Yunzhi powder. Soak the Yunzhi powder in 0.65% sodium chloride aqueous solution for 30 minutes, and then Seal and heat treatment at 65°C for 5 minutes, filter to take filter residue I, add pretreatment solution to filter residue I and soak for 2 hours, boil at 8 MPa for 20 minutes, filter and take filter residue II, add 5 times the volume of The mass fraction is 75% ethanol aqueous solution reflux extraction 2h, total reflux extraction 2 times, filter, take filter residue III, wherein, by weight, the pretreatment solution includes: 0.2 parts of ursolic acid, 0.1 parts of methyl hesperidin, 0.4 parts buccal alcohol, 4 parts guanidine isothiocyanate, 1 part sodium lauryl sulfate, 0.5 parts sodium citrate, 0.5 parts pep...

Embodiment 2

[0028] The extraction method of polysaccharide polysaccharide composition with high sugar content comprises the following steps:

[0029] Step 1. After mixing Yunzhi and dry ice in a weight ratio of 2.0:1, pulverize it to below 120 mesh with a pulverizer to obtain Yunzhi powder. Soak the Yunzhi powder in 0.85% sodium chloride aqueous solution for 40 minutes, and then Seal and heat treatment at 75°C for 10 minutes, filter and take filter residue I, add pretreatment liquid to filter residue I and soak for 4 hours, and slightly boil for 30 minutes under a pressure of 10 MPa, filter and take filter residue II, add 12 times the volume of The mass fraction is 85% ethanol aqueous solution reflux extraction 3h, total reflux extraction 3 times, filter, take the filter residue III, wherein, by weight, the pretreatment solution includes: 0.4 parts of ursolic acid, 0.2 parts of methyl hesperidin, 0.6 parts buccal alcohol, 6 parts guanidine isothiocyanate, 2 parts sodium lauryl sulfate, 1 ...

Embodiment 3

[0035] The extraction method of polysaccharide polysaccharide composition with high sugar content comprises the following steps:

[0036] Step 1. After mixing Yunzhi and dry ice in a weight ratio of 1.8:1, pulverize it to below 120 mesh with a pulverizer to obtain Yunzhi powder. Soak the Yunzhi powder in 0.75% sodium chloride aqueous solution for 35 minutes, and then Seal and heat treatment at 70°C for 8 minutes, filter the filter residue I, add pretreatment solution to the filter residue I and soak for 3 hours, boil it under the pressure of 9MPa for 25 minutes, filter the filter residue II, add 8 times the volume of The mass fraction is 80% ethanol aqueous solution reflux extraction Reflux 2.5h, reflux extraction 3 times in total, filter, take filter residue III, wherein, by weight, the pretreatment solution includes: 0.3 parts of ursolic acid, 0.15 parts of methyl orange peel Glycosides, 0.5 parts of buccal alcohol, 5 parts of guanidine isothiocyanate, 1.5 parts of sodium la...

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Abstract

The invention discloses an extraction method of a coriolus versicolor polysaccharide composition with high sugar content, comprising the following steps: mixing and crushing coriolus versicolor and dry ice, treating successively with a sodium chloride aqueous solution and a pretreatment fluid, filtering and taking filter residue, adding ethanol water into the filter residue and carrying out refluxextraction, filtering, taking filter residue, adding distilled water into the filter residue and carrying out reflux extraction, filtering and taking a filtrate, concentrating the filtrate, adding ethanol, centrifuging and taking a precipitate, washing the precipitate successively with ethanol and acetone, dissolving the washed precipitate with distilled water, sieving with a hollow fiber membrane, collecting a concentrate, centrifuging, filtering and taking a filtrate, and spray-drying the filtrate so as to obtain the coriolus versicolor polysaccharide composition. The content of coriolus versicolor polysaccharide in the coriolus versicolor polysaccharide composition is increased. The invention also discloses application of the coriolus versicolor polysaccharide composition in the preparation of a medicine for treating oral ulcer. The invention has the beneficial effect of providing experimental data base for preparing a medicine for treating oral ulcer from the coriolus versicolor polysaccharide composition.

Description

technical field [0001] The invention relates to the field of versicolor polysaccharides. More specifically, the present invention relates to a method for extracting polysaccharide polysaccharide composition with high sugar content and its application. Background technique [0002] Yunzhi is a precious resource of Chinese herbal medicine in my country. Yunzhi mycelium can produce Yunzhi polysaccharide in vivo and in vitro. Yunzhi polysaccharide is the main active ingredient of Yunzhi. It is a glucan containing β-glycosidic bonds. Yunzhi extract It has the effect of dehumidifying and reducing phlegm, anti-bacteria, mold and virus. It can treat lung diseases, chronic bronchitis, persistent or chronic hepatitis, and can also be used as an immunotherapy drug for patients with liver cancer. At present, the extraction methods of Yunzhi polysaccharides are mainly divided into water extraction method, acid extraction method, and alkali extraction method according to the different ext...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02A61K31/715A61P1/02
CPCC08B37/0003A61K31/715A61P1/02C08B37/0024
Inventor 陈德兴
Owner 陈德兴
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