Carbon dioxide concentration control method and device for microfluidic cell culture

Abstract

The invention discloses a carbon dioxide concentration control method and device for microfluidic cell culture, which can generate a carbon dioxide concentration environment for microfluidic cell culture experiments. The first piston controls the gas in the pure carbon dioxide gas storage bottle to pass through the first valve. To flow into the gas mixing chamber, the second piston controls the gas between the outside and the gas mixing chamber to flow bidirectionally through the second valve, the first piston and the second piston have the same structure, and the cell culture chamber communicates with the gas mixing chamber through a circulating ventilation pipe; Decompose the volume of pure carbon dioxide that needs to be replenished as a weight value, determine the four unit inflation volumes, calculate the number of times of dosing corresponding to each unit inflation volume, fill in pure carbon dioxide in turn, and control the electrification of the piston excitation coil in the second piston. Air is filled or exhausted into the gas mixing chamber; the entire gas mixing and gas use are carried out in a closed space, and the concentration of carbon dioxide is directly used for microfluidic cell culture to improve the success rate of cell culture.

Description

technical field [0001] The invention relates to the field of microfluidic cell culture, in particular to a method and device for controlling carbon dioxide concentration for microfluidic cell culture, which can generate a carbon dioxide concentration environment that can be used in microfluidic cell culture experiments. Background technique [0002] As an important technology in the fields of medical treatment, pharmacy, and biological research, cell culture has high requirements no matter what type of cell it is. Conditions such as stable carbon dioxide levels, constant pH, and high relative saturation humidity. Among them, the control of the carbon dioxide concentration level is more difficult, because the cells are accompanied by the production or consumption of carbon dioxide during the culture process, and in addition, there are artificial opening and closing of the incubator during the culture process, which requires a dynamic adjustment of carbon dioxide concentration...

AI Technical Summary

Problems solved by technology

During the working process of the CO2 box, the gas cylinder is used to replenish the working chamber to maintain a stable CO2 concentration; however, this method uses a combination of a flow meter and a valve to control the amount of inflation, due to the delay of the valve switch The problem leads to low control accuracy and increases the cost of the device

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