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A magnetic carbon nanotube and its extraction method for clenbuterol in pork

A magnetic carbon nanotube, clenbuterol technology, applied in solid solvent extraction, chemical instruments and methods, carbon compounds, etc., can solve the problems of large consumption of organic solvents, only one use, and low specificity, etc. Beneficial for consistency, less interference, and simple preparation method

Active Publication Date: 2021-04-27
JILIN INST OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complexity of the ingredients in the food, and the content of clenbuterol to be detected is usually at trace levels, in order to improve the detection accuracy and sensitivity, pretreatment of the pork samples to be tested is required
Due to the problems of large consumption of organic solvents, low specificity, high price, and only one-time use of traditional solid-phase extraction cartridges, it is necessary to develop a new type of solid-phase extraction column with strong enrichment ability for clenbuterol, simple preparation method, and low price. Extraction material

Method used

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  • A magnetic carbon nanotube and its extraction method for clenbuterol in pork
  • A magnetic carbon nanotube and its extraction method for clenbuterol in pork
  • A magnetic carbon nanotube and its extraction method for clenbuterol in pork

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Embodiment 1

[0040] This embodiment provides a magnetic carbon nanotube and its preparation method, comprising the following steps: weighing 1.668g FeSO 4 ·7H 2 O and 0.727 g L-cysteine ​​(Cys, HSCH 2 CH(NH 2 ) CO 2H), respectively dissolved in 30ml ultrapure water, after complete dissolution, quickly mix the two solutions, then add 48mL 1mol / L NaOH solution to it, ultrasonically and continuously stir for 10min, then add 0.3g carbon nanotube (CNT), Sonicate and stir continuously for 20 minutes, wash with ultrapure water and absolute ethanol until clean, and vacuum dry at 60°C for 4 hours to prepare magnetic carbon nanotubes Fe 3 o 4 @Cys @CNT.

[0041] In the following, the magnetic Fe is characterized by infrared spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM) and other methods. 3 o 4 Physical and chemical properties of @Cys@CNT.

[0042] Depend on figure 1 It can be seen that, as shown in a, at 590cm -1 There is a strong absorption peak, which is t...

Embodiment 2

[0045] This embodiment utilizes the magnetic carbon nanotube Fe that embodiment 1 prepares 3 o 4 The method for @Cys@CNT to extract clenbuterol from pork includes the following steps:

[0046] Refer to GB / T5009.192-2003 first method to extract the clenbuterol in the pork sample to be tested to obtain 25mL of the sample solution containing clenbuterol, then adjust the pH to 7 with 1mol / L sodium hydroxide solution, if there is precipitation , and then centrifuged at 4500min / r for 10min to remove the precipitate, and 8g of sodium chloride was added to the supernatant to obtain an extract;

[0047] Set the volume to 50mL with the above-mentioned extract, take 10mL of the above-mentioned extract into a brown vial, and then add 20mg of the magnetic carbon nanotube Fe 3 o 4 @Cys@CNT, vortex extraction for 10min, magnetic separation, discard the supernatant, add 3mL acetic acid:methanol=15%:85% eluent; again vortex elution for 10min, magnetic separation, after magnetic separation a...

Embodiment 3

[0049] Investigate the effect of different alkali solution concentrations on the magnetic carbon nanotubes Fe 3 o 4 Effect of @Cys@CNT on extraction efficiency.

[0050] Prepare the magnetic carbon nanotube Fe according to the method described in Example 1 3 o 4 @Cys@CNT, the difference is that the concentration of NaOH solution is 1mol / L, 1.5 mol / L, and 2 mol / L respectively. Depend on image 3 It can be seen that in the preparation of magnetic carbon nanotubes Fe 3 o 4 When @Cys@CNT, when the concentration of the added NaOH solution is between 1~2mol / L, the extraction efficiency does not change with the change of the concentration. Therefore, in line with the principle of saving, when preparing magnetic carbon nanotubes, should Choose 1 mol / L NaOH solution.

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Abstract

The invention discloses a magnetic carbon nanotube and its extraction method for clenbuterol in pork. The invention uses FeSO 4 ·7H 2 Magnetic carbon nanotubes Fe 3 o 4 @Cys @CNT. Using the magnetic carbon nanotube Fe 3 o 4 @Cys@CNT is used as an adsorbent to extract clenbuterol (ractopamine and clenbuterol hydrochloride) in pork, which realizes the effective enrichment and separation of clenbuterol in pork samples to be tested, which can greatly improve the concentration of clenbuterol in existing pork Sensitivity of clenbuterol detection method.

Description

technical field [0001] The invention belongs to the technical field of food detection, in particular to a magnetic carbon nanotube and a method for extracting clenbuterol from pork. Background technique [0002] Clenbuterol (ractopamine and clenbuterol) is an asthma medicine. The drug is a moderately accumulative drug, and its accumulation in animal tissues is related to its dose and administration duration, and its residual amount gradually decreases with the prolongation of the withdrawal period. Feeding clenbuterol can increase the lean meat rate of pigs and bring more economic value, but eating pork containing clenbuterol has dangerous side effects on the human body. In China, the first reported clenbuterol poisoning incident was in 1998 It was caused by the supply of live pigs to Hong Kong in 2009, and such incidents have often occurred since then. At present, clenbuterol has been banned in our country. [0003] The detection method of clenbuterol has gas chromatogra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/08B01D11/02C01B32/168
CPCB01D11/02C01B32/168G01N30/02G01N30/06G01N30/08G01N2030/062
Inventor 连丽丽张鑫洋吕进义娄大伟高文秀张浩王希越马先红
Owner JILIN INST OF CHEM TECH
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