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Multichannel liquid drop generation device and method based on macroporous perfusion microspheres

A droplet generation, multi-channel technology, used in chemical instruments and methods, laboratory containers, laboratory utensils, etc., can solve the problem of inability to achieve multi-channel parallelism, affecting the uniformity and consistency of droplets, instability, etc. problems, to achieve the effect of easy batch parallel droplet preparation, increased droplet generation flux, and increased density

Active Publication Date: 2018-12-04
晋江精纯科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in practical applications, the power source (pressure pump, syringe pump, etc.) of the dispersed phase and the continuous phase is prone to instability during long-term operation, thereby affecting the uniformity and consistency of the droplet size; another shortcoming It is impossible to achieve high-density multi-channel parallelism, and the droplet volume is at the nanoliter level or smaller. How to achieve large-scale preparation is also an important challenge

Method used

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  • Multichannel liquid drop generation device and method based on macroporous perfusion microspheres
  • Multichannel liquid drop generation device and method based on macroporous perfusion microspheres
  • Multichannel liquid drop generation device and method based on macroporous perfusion microspheres

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Example 1: Construction of a multi-channel droplet generation device with an inner diameter of 100 μm

[0025] see figure 1 and 2 , intercept a 10cm capillary 3 with an inner diameter of 100 μm, press the dispersed phase 10 into the parallel capillary 3 with a syringe pump 5, fix a large-pore perfusion silicon ball 7 with an outer diameter of 100 μm on the port of the capillary 3 based on the cornerstone effect, and place the capillary 3. One end of the macroporous perfusion microsphere is put into the container 8 filled with fluorocarbon-40 (FC-40) 4 continuous phase, and the other end of the capillary 3 is connected to the syringe 1 by using a PEEK hose 2, and the water / methanol ( 1 / 1, v / v) is the dispersed phase 10, and manually pressing 1 mL of the continuous phase 9 into the capillary 3 can quickly generate a large number of droplets 11.

Embodiment 2

[0026] Example 2: Construction of 8 parallel multi-channel capillary droplet generating devices

[0027] Eight quartz capillary tubes 3 with a length of 10 cm and an inner diameter of 100 μm were cut. Using the method in Example 1, a perfusion silicon sphere with an outer diameter of 100 μm was fixed at the end of each capillary based on the cornerstone effect, and one end of the capillary with a large-pore perfusion microsphere was placed in a container filled with FC-40 4 continuous The other end is connected to the syringe with a PEEK hose 2, and four three-way 6 devices are used to divide the dispersed phase into 8 channels (such as image 3 ). Using water / methanol (1 / 1, v / v) as the dispersed phase, use the syringe pump 5 to press the dispersed phase into 8 parallel capillary tubes 3 at a flow rate of 500 μL / min, realizing a multi-channel multi-channel capillary droplet generating device in parallel build.

Embodiment 3

[0028] Example 3: Generation of Highly Uniform Size Droplets

[0029] Using the droplet generation device built in Example 1, FC-40 was used as the continuous phase, water / methanol (1 / 1, v / v) was used as the dispersed phase, and the dispersed phase was injected at a flow rate of 50 μL / min using a precision syringe pump. Continuous phase, prepare droplet, and observe under microscope, measure the size of droplet, by measuring the diameter of 50 droplets, calculate the CV value of gained droplet to be 3%, show that gained droplet size is highly uniform ( Figure 4 ).

[0030] The present invention is composed of capillary, macroporous perfusion microspheres, liquid delivery device and droplet collection device: macroporous perfusion microspheres are fixed at the capillary port, then the capillary is placed under the liquid surface of the continuous phase, and the dispersed phase passes through the capillary at a certain flow rate Inject into the continuous phase. When the disp...

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Abstract

Relating to the field of microfluidic liquid drop generation, the invention provides a multichannel liquid drop generation device and method based on macroporous perfusion microspheres. The liquid drop generation device is equipped with capillary tubes, macroporous perfusion microspheres, a liquid conveying unit and a liquid drop collection unit. The macroporous perfusion microspheres are fixed atthe ports of the capillary tubes, and have multiple through pore channels. The capillary tubes are disperse phase channels. The liquid conveying unit is used for conveying a disperse phase. The liquid drop collection unit is used for holding a continuous phase and collecting liquid drops. Based on s cornerstone effect, perfusion silicon spheres are fixed at the ports of the capillary tubes, thenone ends of the capillary tubes with macroporous perfusion microspheres are put into a container holding a fluorocarbon-40 continuous phase, the other ends of the capillary tubes with macroporous perfusion microspheres are connected to a syringe by a PEEK hose, water / methanol are adopted as the disperse phase, and the continuous phase is pressed into the capillary tubes, thus generating a large number of liquid drops rapidly.

Description

technical field [0001] The invention relates to the field of microfluidic droplet generation, in particular to a multi-channel droplet generation device and method based on macroporous perfusion microspheres. Background technique [0002] Microfluidic droplet preparation technology is a new micro droplet preparation and operation technology developed in recent years. It uses two immiscible liquid phases to divide the fluid into a certain volume of liquid by using shear force and surface tension. drop. Due to the advantages of tiny droplets in mass transfer, heat transfer, material exchange, and quantitative control, microfluidic droplet technology has attracted extensive attention and research in recent years. [0003] At present, the preparation of droplets is mainly through the crossing and relative movement between the continuous phase and the dispersed phase to form droplets, such as using a pressure pump to pass the dispersed phase and the continuous phase through the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
CPCB01L3/5027
Inventor 王晓飞张博
Owner 晋江精纯科技有限公司