Use of tamoxifen and a method for constructing a cleft palate animal model

Abstract

The invention especially relates to application of tamoxifen and a method for constructing a cleft palate animal model, belonging to the field of genetic engineering technology. Research results of the invention show that a female mouse (Tgfbr1<f / f>K14Cre+) is mated with a male mouse (Tgfbr1<f / f>K14Cre-), or a female mouse (Tgfbr1<f / f>K14Cre-) is mated with a male mouse (Tgfbr1<f / f>K14Cre+), and Tgfbr1 gene knockdown can be performed on a fetal mouse thereof under the induction of tamoxifen; so the cleft palate animal model is constructed. In the mouse constructed by using the method of the invention, Tgfbr1 genes in palatal epithelial cells are knocked out, and interference of other factors in the cleft palate model is reduced.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to the application of tamoxifen and a method for constructing a cleft palate animal model. Background technique [0002] Since the completion of the Human Genome Project, life has entered the scientific research into the post-genome era, and the core work of the post-genome era is to study the function of genes. At present, the most effective method for studying gene function is gene targeting (gene knockout and gene knockin) technology. Gene targeting technology is a new technology developed in the field of life science research in recent years. It is developed on the basis of embryonic stem cell technology and homologous recombination technology. Alter the phenotype of a genetically modified animal to gain information about gene function. There is another important use of genetic technology, making animal models of human diseases. [0003] Studies have now shown tha...

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