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Use of tamoxifen and a method for constructing a cleft palate animal model

A technology of tamoxifen and construction method, applied in the field of construction of cleft palate animal model

Inactive Publication Date: 2020-12-22
BEIJING STOMATOLOGY HOSPITAL CAPITAL MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cleft palate model of mice with K14 conditional knockout of the Tgfbr1 gene established by Dudas et al. has been reported so far. The cleft palate phenotype appears at the back of the soft palate. Gene function complementation is strong and the expression of deformity is reduced

Method used

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  • Use of tamoxifen and a method for constructing a cleft palate animal model
  • Use of tamoxifen and a method for constructing a cleft palate animal model
  • Use of tamoxifen and a method for constructing a cleft palate animal model

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Embodiment 1

[0033] 1. Experimental animals and materials

[0034] 1.1 Experimental animals

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Abstract

The invention especially relates to application of tamoxifen and a method for constructing a cleft palate animal model, belonging to the field of genetic engineering technology. Research results of the invention show that a female mouse (Tgfbr1<f / f>K14Cre+) is mated with a male mouse (Tgfbr1<f / f>K14Cre-), or a female mouse (Tgfbr1<f / f>K14Cre-) is mated with a male mouse (Tgfbr1<f / f>K14Cre+), and Tgfbr1 gene knockdown can be performed on a fetal mouse thereof under the induction of tamoxifen; so the cleft palate animal model is constructed. In the mouse constructed by using the method of the invention, Tgfbr1 genes in palatal epithelial cells are knocked out, and interference of other factors in the cleft palate model is reduced.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to the application of tamoxifen and a method for constructing a cleft palate animal model. Background technique [0002] Since the completion of the Human Genome Project, life has entered the scientific research into the post-genome era, and the core work of the post-genome era is to study the function of genes. At present, the most effective method for studying gene function is gene targeting (gene knockout and gene knockin) technology. Gene targeting technology is a new technology developed in the field of life science research in recent years. It is developed on the basis of embryonic stem cell technology and homologous recombination technology. Alter the phenotype of a genetically modified animal to gain information about gene function. There is another important use of genetic technology, making animal models of human diseases. [0003] Studies have now shown tha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/138A01K67/027
CPCA01K67/0275A01K2217/054A01K2227/105A61K31/138
Inventor 杜娟孙林栋刘惠娜
Owner BEIJING STOMATOLOGY HOSPITAL CAPITAL MEDICAL UNIV
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