Check patentability & draft patents in minutes with Patsnap Eureka AI!

An antibacterial/anti-osteosarcoma/bone-promoting multifunctional titanium-based implant material and its preparation method

A multi-functional, implantable material technology, applied in pharmaceutical formulations, medical science, prostheses, etc., can solve the problems of free osteosarcoma cell residues, tumor recurrence, damage to osseointegration, etc., and achieve good clinical application prospects and repeatability Good, easy to operate effect

Active Publication Date: 2020-09-01
CHONGQING UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In addition, there are many free osteosarcoma cells left after osteosarcoma resection, which may also cause tumor recurrence and damage the process of osseointegration

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • An antibacterial/anti-osteosarcoma/bone-promoting multifunctional titanium-based implant material and its preparation method
  • An antibacterial/anti-osteosarcoma/bone-promoting multifunctional titanium-based implant material and its preparation method
  • An antibacterial/anti-osteosarcoma/bone-promoting multifunctional titanium-based implant material and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A method for preparing an antibacterial / anti-osteosarcoma / bone-promoting multifunctional titanium-based implant material, characterized in that it comprises the following steps:

[0051] 1) Construct a strontium ion-doped hydroxyapatite (Sr-HA) porous coating on the surface of titanium by micro-arc oxidation treatment;

[0052] 1.1) Immerse the pretreated titanium foil in the electrolyte, react under voltage, and obtain the sample after 10 minutes;

[0053] Include strontium acetate, calcium acetate, beta glycerophosphate sodium and water in the electrolyte;

[0054] The concentration of strontium acetate is 0.05M; the concentration of calcium acetate is 0.15M; the concentration of sodium beta glycerophosphate is 0.2M;

[0055]Said voltage is 550V;

[0056] The pretreatment process is as follows: after cutting 20 pieces of pre-cut titanium foils (1×1 cm), they are washed with ethanol, acetone and deionized water for 10 minutes in sequence; they are dried for later use...

experiment example 1

[0070] Bacterial morphology and antibacterial efficiency detection on the surface of the sample

[0071] Use an initial concentration of 2 x 10 6 Staphylococcus aureus (S.aureus, ATCC29213) and Escherichia coli (E.coli, ATCC2592) bacteria liquid per mL were used for bacterial experiments; specifically, the following steps were included:

[0072] 1) Two kinds of bacteria (1 mL) were inoculated on the surface of different materials for 6 hours (37°C), and the surface-adhering bacteria were pre-fixed (40 min) with a 4% paraformaldehyde solution;

[0073] 2) Then use gradient ethanol solution to dehydrate the bacteria, and finally observe the bacteria samples with SEM;

[0074] In addition, after 6 and 24 hours of bacterial inoculation, the adherent bacteria on the surface of different materials were separated and collected by ultrasound, and then the antibacterial efficiency of different samples was characterized by the bacteria-coated plate method.

[0075] Experimental result...

experiment example 2

[0083] Detection of osteosarcoma cell proliferation on material surface

[0084] Use an initial concentration of 2 x 10 4 Saos-2 cells per well were inoculated on the surface of the material, and after 3 days of culture, the proliferation of osteosarcoma cells (Saos-2 cells) in each group was explored by the active fluorescein diacetate (FDA) staining experiment;

[0085] In the test, in order to prepare FDA stained samples, 2 μL of FDA dye solution (10 μg / mL) was added to the cell culture medium, and after the cells were incubated for 10 minutes, the remaining dye solution was removed with PBS buffer; finally, the staining was performed using a fluorescence microscope (FM). The live cells of Saos-2 were observed and analyzed;

[0086] Experimental results such as Figure 6 As shown, specifically:

[0087] Such as Figure 6 Shown are the FDA fluorescence images of osteosarcoma cells (Saos-2 cells) and osteoblasts (osteoblasts) after normal or pre-soaked treatment for 1 day...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
volumeaaaaaaaaaa
Login to View More

Abstract

The invention discloses an anti-bacteria / anti-osteosarcoma / osteogenesis promotion multifunctional titanium-based implant material and a preparation method thereof. The preparation method includes thefollowing steps: constructing a strontium ion-doped hydroxyapatite porous coating on the surface of a titanium material by micro-arc oxidation treatment; then placing a sample in a high-pressure reaction kettle containing 2,5-dihydroxyterephthalic acid and magnesium nitrate, performing a reaction for 24 h at 125 DEG C, and obtaining a metal organic skeleton coating on the surface. The obtained modified sample of Mg-MOF74 / Sr-HA composite coating exhibits superior properties of anti-bacteria, anti-osteosarcoma and osteogenesis promotion, and had good clinical application prospects.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a preparation method of an antibacterial / anti-osteosarcoma / bone-promoting multifunctional titanium-based implant material. Background technique [0002] In recent years, bacterial infection has been shown to be the second leading cause of orthopedic implant failure. Studies have shown that 4-6 hours after infection is a critical period for bacterial treatment. In this stage, the invading bacteria will attach to the surface of the implant and proliferate in large quantities, and then form a dense biofilm layer to protect the internal bacteria from antibacterial substances. early integration. [0003] In addition, free osteosarcoma cells often remain after osteosarcoma resection, and such cells may also cause tumor recurrence and impair the osseointegration process. [0004] Therefore, for patients with osteosarcoma resection or bacterial secondary revision surgery, the devel...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/06A61L27/56A61L27/58A61L27/54A61L27/50A61L27/32A61L27/28
CPCA61L27/06A61L27/28A61L27/32A61L27/50A61L27/54A61L27/56A61L27/58A61L2300/102A61L2300/404A61L2300/412A61L2300/61A61L2400/18A61L2420/02A61L2420/08A61L2430/02
Inventor 蔡开勇沈新坤张杨杨
Owner CHONGQING UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com