A kind of ph-sensitive Bacillus subtilis bacterial agent

A technology of Bacillus subtilis and spores, applied in the directions of bacteria, biocides, animal repellents, etc., can solve problems such as unfavorable crop growth, fertilizer loss, reducing fertilizer utilization efficiency, etc., to improve nutrient conditions and nutrient supply conditions, inhibit activities, the effect of improving soil salinization problems

Active Publication Date: 2020-11-20
河北丰农有机肥制造有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Salinized soil is not conducive to the growth of crops, and a large number of hydrogen ions in acidic soil lead to the loss of some fertilizers, which greatly reduces the utilization efficiency of fertilizers
For the solution to the above problems, there is no effective means to propose

Method used

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  • A kind of ph-sensitive Bacillus subtilis bacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1, the preparation of pH-sensitive bacillus subtilis inoculum

[0038] (1) Bacillus subtilis surface coated with dopamine

[0039] Weigh 1.5g Tris and place it in a beaker, add about 900mL of deionized water, and adjust the pH of the Tris-HCl solution to 8-8.5 with 6M HCl; -HCl solution, and dilute the solution to 1 L; add 5 g of dopamine hydrochloride, stir at room temperature for more than 2 hours, and suction filter to obtain Bacillus subtilis coated with dopamine on the surface.

[0040] (2) Coating of pH-sensitive polymer (Pdop-g-PAA-co-HMAm)

[0041]Weigh 2.0 g of NaOH solid into 20 mL of ultrapure water, and stir magnetically at room temperature until NaOH is completely dissolved. After the solution was cooled to room temperature, 3.6 g of acrylic acid (AA) was added dropwise thereto, and magnetically stirred at room temperature for 1 h (covered with tin foil to avoid light), to obtain a 2.5 mol / L sodium acrylate solution (SA).

[0042] Put 5-10 g o...

Embodiment 2

[0043] Embodiment 2, live bacteria release experiment of pH-sensitive Bacillus subtilis bacterial agent

[0044] Get pH-sensitive Bacillus subtilis inoculum 6g prepared according to the method described in Example 1, divide it into 3 parts on average, place it in sterile water with a pH value of 5, 7, and 9 for 1 day, and then detect the different pH values. In some cases, the release amount of live bacteria of the pH-sensitive bacterial agent.

[0045] Plate detection method to detect the release of viable bacteria detection method:

[0046] 1) Prepare a solution according to the mass volume ratio of the placed sample: sterile water at a ratio of 1:10, and filter the solution through a 3-micron filter membrane to filter out the dopamine attached to the surface of the unreleased live bacteria Bacteria particles and bacteria particles coated with polymer molecules;

[0047] 2) Collect the filtrate, add glass beads, let it stand for 20 minutes, and shake it on a rotary shaker ...

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Abstract

The invention provides a pH sensitive bacillus subtilis fungicide. The fungicide comprises bacillus subtilis and / or spore, dopamine or salt thereof and pH sensitive polymer macromolecules. The dopamine is attached to the surface of the bacillus subtilis, and the surface of the dopamine is coated with the pH sensitive polymer molecules, so that the pH sensitive bacillus subtilis fungicide capable of adjusting the release amount of live bacteria wrapped therein according to the pH vale of the environment can be prepared. In the salinization soil, by applying the pH sensitive bacillus subtilis fungicide provided in the invention, the live bacteria can be rapidly released, so that the soil salinization problem of the soil can be rapidly improved; and by applying the pH sensitive bacillus subtilis fungicide provide in the acidified soil, the release rate of the live bacteria in the fungicide can be effectively retarded, the loss of organic fertilizer generated by the decomposition of live bacteria in the acid soil can be prevented, and the utilization efficiency of the fertilizer can be improved.

Description

technical field [0001] The invention belongs to the technical field of fertilizer development, and in particular relates to a pH-sensitive Bacillus subtilis bacterial agent. Background technique [0002] The use of agricultural microbial agents greatly reduces the amount of chemical fertilizers and the pollution they cause. As a kind of biological fertilizer, agricultural microbial agent can improve the nutritional conditions and nutrient supply status of crops through the life activities of microorganisms, accelerate the absorption and transformation of nutrients, and inhibit the activities of harmful microorganisms. It is widely used in modern agricultural production. use. [0003] Bacillus subtilis is a kind of Bacillus genus, which is an endogenous rod-shaped bacterium resistant to reverse spores. It is widely found in nature such as soil, lakes, oceans, and the surfaces of animals and plants. It has no pathogenicity itself. As one of the biocontrol bacteria for plant ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22C09K17/40A01P21/00C12R1/125C09K101/00C09K109/00
CPCA01N63/00C09K17/40C09K2101/00C09K2109/00C12N1/20
Inventor 温岚
Owner 河北丰农有机肥制造有限公司
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