Reference substance for testing specific wavelength of full automatic biochemical analyzer, and application of reference substance
A wavelength-specific, fully automatic technology, applied in biological testing, color/spectral characteristic measurement, material inspection products, etc., can solve the problems of lack of photometric wavelength standards and poor accuracy, so as to reduce the impact and achieve accurate test results Effect
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Embodiment 1
[0040] Fully automatic biochemical analyzer photometric wavelength standard: composed of polylactic acid microspheres, preservatives and buffer; the diameter of the microspheres is 200nm, and the concentration is 0.1%; the buffer is 20mmol / L phosphate buffer; the preservative is Proclin 300 at a concentration of 0.1%.
[0041] The preparation method of the photometric wavelength standard of the above-mentioned automatic biochemical analyzer: use a pipette to accurately measure 150 μL of the microsphere solution with a concentration of 10%, and add it to 14.85 mL of phosphate buffer (20 mmol / L) to obtain a concentration of 0.1 % of the microsphere solution, add 15 μL of Proclin 300, which is the standard substance, recorded as 200-0.1%.
[0042] Take the standard substance of Example 1 with a volume of 10 mL, use a Shimadzu UV-Vis spectrophotometer to perform full-spectrum scanning (190nm-900nm), detect the absorbance of the microspheres at 190nm-900nm, and draw an absorbance l...
Embodiment 2
[0046] Fully automatic biochemical analyzer photometric wavelength standard: composed of polylactic acid microspheres, preservatives and buffer; the diameter of the microspheres is 200nm, and the concentration is 0.05%; the buffer is 20mmol / L phosphate buffer; the preservative is Proclin 300 at a concentration of 0.1%.
[0047] The preparation method of the above-mentioned fully automatic biochemical analyzer photometric wavelength standard substance standard substance: use a pipette to accurately measure 75 μL of the microsphere solution with a concentration of 10%, add it to 14.925mL of phosphate buffer (20mmol / L), and obtain the concentration To a 0.05% microsphere solution, add 15 μL of Proclin 300, which is the standard substance, recorded as 200-0.05%.
[0048] Take the standard substance of Example 2 with a volume of 10 mL, use a Shimadzu UV-Vis spectrophotometer to perform full-spectrum scanning (190nm-900nm), detect the absorbance of the microspheres at 190nm-900nm, a...
Embodiment 3
[0052] Fully automatic biochemical analyzer photometric wavelength standard: composed of polylactic acid microspheres, preservatives and buffer, the diameter of the microspheres is 200nm, and the concentration is 0.025%; the buffer is 20mmol / L phosphate buffer; the preservative is Proclin 300 at a concentration of 0.1%.
[0053] The preparation method of the photometric wavelength standard of the above-mentioned automatic biochemical analyzer: use a pipette to accurately measure 37.5 μL of the microsphere solution with a concentration of 10%, add it to 14.9625 mL of phosphate buffer (20 mmol / L), and obtain the concentration To a 0.025% microsphere solution, add 15 μL of Proclin 300, which is the standard substance, recorded as 200-0.025%.
[0054] Take the standard substance of Example 3 with a volume of 10 mL, use a Shimadzu UV-Vis spectrophotometer to perform full-spectrum scanning (190nm-900nm), detect the absorbance of the microspheres at 190nm-900nm, and draw an absorbanc...
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Abstract
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