Plaque removal device for virus isolation and purification

Abstract

The invention discloses a plaque removal device for virus isolation and purification. The plaque removal device comprises a removal pipe and an outer canula, a support plate is fixed at the lower endof the outer canula, the lower end of the support plate is provided with the non-slip protruding blocks with array distribution, and an inner support rod passes through the upper end of the outer canula, the inner support rod and the outer canula are fixed by a fixing pin, the inner support rod is provided with limiting holes with array distribution by cooperating with the outer canula, the upperend of the inner support rod is fixed with a battery, a second gooseneck pipe is arranged at a left end of the battery, the removal pipe is disposed on the left side of the battery, and the removal pipe is connected to the battery through the second gooseneck pipe. According to the plaque removal device for virus isolation and purification, arrangement of a vent hole and a sealing cover can keep communication of inside of the removal pipe and the atmosphere, which cannot cause the plaque to fall off from a moving head, the setting of a LED illuminating lamp can ensure the light when plaque isremoved, the setting of a heating guide wire can ensure the plaque temperature environment, and the setting of the second gooseneck pipe is convenient for hand-held removal of plaque.

Description

technical field [0001] The invention relates to a plaque taking device, in particular to a plaque taking device for virus separation and purification. Background technique [0002] Phage plaque is a transparent circle visible to the naked eye formed on a plate containing recipient bacteria after phage infection of sensitive host bacteria. On the surface of a solid medium coated with sensitive host cells, if a dilution of the corresponding phage is inoculated, each phage particle first infects and lyses one cell, and then repeatedly infects and lyses a large number of surrounding cells with this as the center. As a result, a phage plaque with a certain shape, size, edge and transparency will be formed on the lawn. [0003] In the field of microbiology, in the process of virus isolation and purification, it is necessary to obtain phage plaques first. The traditional method is to use a pipette tip to dig or use an inoculation loop to pick up. The tip of the pipette is quick t...

AI Technical Summary

Problems solved by technology

The tip of the pipette is quick to dig out, but the process is cumbersome and the cost is high; while the hooking of the inoculation loop reduces the cost, but the inoculation loop will cause damage to the phage plaque during the hooking process, and the phage plaque cannot be completely obtained, and the damaged phage Plaque is also susceptible to bacterial contamination

At the same time, the existing equipment also cannot guarantee the temperature environment and light intensity when the plaque is removed.

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