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Application of Bletilla striata oligosaccharides in improving intestinal microecology

A technology of intestinal microecology and Bletilla striata oligosaccharide, which can be used in medical preparations containing active ingredients, non-central analgesics, extracellular fluid diseases, etc. And other issues

Active Publication Date: 2020-11-06
HUBEI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the drugs used to treat enteritis are antibiotics and aminosalicylic acids that directly act on the intestinal mucosa. Although they have a therapeutic effect on the disease, they cause intestinal flora disturbance, drug resistance, drug dependence, liver and kidney diseases to varying degrees. side effects such as damage

Method used

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  • Application of Bletilla striata oligosaccharides in improving intestinal microecology
  • Application of Bletilla striata oligosaccharides in improving intestinal microecology
  • Application of Bletilla striata oligosaccharides in improving intestinal microecology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Preparation of Bletilla striata oligosaccharides by strong acid method

[0038] In this embodiment, a method for preparing bletilla striata oligosaccharides by strong acid hydrolysis is provided, and the specific implementation steps are as follows:

[0039] (1) Take 25g of Bletilla striata tubers and grind them into 80-mesh powder, add 500ml of water to mix, dissolve and extract at 80°C for 8 hours to obtain an extract;

[0040](2) Filter the extract described in step (1), obtain the filtrate after intercepting the precipitate, and distill under reduced pressure to obtain a concentrated solution of 50ml;

[0041] (3) Add 150 ml of 95% ethanol to the concentrated solution in step (2), and let stand overnight for 12 hours.

[0042] (4) Filter the concentrated solution after step (3) standing overnight, and obtain the precipitate after intercepting;

[0043] (5) After the precipitate described in step (4) is redissolved, add trifluoroacetic acid (final concent...

Embodiment 2

[0044] Example 2 Preparation of Bletilla striata oligosaccharides by enzymatic hydrolysis

[0045] In this embodiment, a method for preparing bletilla striata oligosaccharides by polysaccharide hydrolase is provided, and the specific implementation steps are as follows:

[0046] (1) Take 40g of Bletilla striata striata powder, add 600ml of water to mix, dissolve and extract at 40°C for 12 hours to obtain an extract;

[0047] (2) Filter the extract described in step (1), obtain the filtrate after intercepting the precipitate, and distill under reduced pressure to obtain a concentrated solution of 60ml;

[0048] (3) Add 200 ml of 90% ethanol to the concentrated solution in step (2), and let stand overnight.

[0049] (4) Filter the concentrated solution after step (3) standing overnight, and obtain the precipitate after intercepting;

[0050] (5) Redissolve the precipitate in step (4) with 50ml of water, add 1g of mannanase, hydrolyze at 40°C for 4 hours at pH 6.8, add a certai...

Embodiment example 1

[0053] Implementation Case 1 Bletilla striata oligosaccharides reduce DSS-induced colitis

[0054] (1) In this embodiment, in order to evaluate the improvement effect of bletilla striata oligosaccharides on colitis mice, the specific implementation steps are as follows:

[0055] (2) 4-week-old C57BL / 6J mice were taken, fed with normal standard feed and sterile drinking water, and the mice were adapted to the new environment for 1 week.

[0056] (3) To start the experiment, the mice were treated with 3.5% dextran sodium sulfate (DSS) by intragastric administration, and at the same time, 2% Bletilla striata oligosaccharides were administered by intragastric administration. The processing time is 10 days.

[0057] (4) Detect drinking water, diet, body weight, and stool fineness during treatment.

[0058] (5) Summarize the disease scores of the mice and perform histological scoring.

[0059] The experimental results are attached image 3 .

[0060] The results showed that aft...

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Abstract

The invention discloses application of tuber bletillae oligosaccharide for improving the intestinal tract micro-ecology. The degree of polymerization of the tuber bletillae oligosaccharide is 2-20, and a molecular weight range is 200-5000Da. By use of the tuber bletillae oligosaccharide, the immunoreaction of acute colitis can be lowered, the typical symptom of the colitis is inhibited, intestinalflora composition is optimized, and an intestinal barrier function is enhanced. The tuber bletillae oligosaccharide and the composite thereof can be used for improving the disease prevention and curing of intestinal tract micro-ecology disorder caused by various reasons.

Description

technical field [0001] The invention specifically relates to the application of bletilla striata oligosaccharides in improving intestinal microecology. Background technique [0002] Intestinal microecology refers to a huge ecosystem formed by the host intestine and its adjacent microorganisms. Intestinal bacteria account for 1 / 3 of the intestinal content, with a large number and variety, each with different characteristics, and different effects on the host. Intestinal bacteria that exceed the number of host cells by 10 times are stabilizing the intestinal barrier It plays an important role in regulating metabolism and participating in immunity. Existing evidence shows that the disturbance of intestinal flora can lead to obesity, diabetes, inflammatory bowel disease, depression and other diseases. The damage of intestinal barrier function will further deteriorate the composition of intestinal flora. There is a close relationship between the intestinal flora and the host, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/702A61K31/715A61K31/606A61P7/02A61P1/00A61P29/00A61P1/04
CPCA61K31/606A61K31/702A61K31/715A61P1/00A61P1/04A61P7/02A61P29/00A61K2300/00
Inventor 刘洪涛郑军平杨化冰邹小娟孔明望张志刚夏慧陈会敏
Owner HUBEI UNIV OF CHINESE MEDICINE
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