Magnetic bead mixer for biology, and application thereof

Abstract

The invention discloses a magnetic bead mixer for biology, and an application thereof, and belongs to the field of biology. The mixer comprises a driving device, a transmission device, a magnetic beadcontainer carrying device, and a control device provided with the control software; the driving device, the transmission device and the magnetic bead container carrying device are sequentially connected from bottom to top, the control device is respectively connected with the driving device, the transmission device and the magnetic bead container carrying device, and the control device controls the driving device and / or the transmission device and / or the magnetic bead container carrying device to cooperatively work; the driving device and the transmission device are sleeved; the driving device is used for providing power for the mixer; the transmission device is used for transmitting the driving power to the magnetic bead container carrying device; and the magnetic bead container carryingdevice is used for converting the transmitted driving force into vortex vibration of the mixer in order to realize the uniform dispersion of magnetic beads. The magnetic bead mixer can keep the concentration distribution of a magnetic bead suspension uniform, ensures the distribution of an equal volume of magnetic beads in all reaction holes, and ensures the accuracy of experiments.

Description

Technical field [0001] The invention relates to a magnetic bead mixer for biology and its application, and belongs to the field of biology. Background technique [0002] In the research and production of molecular biology, it is often necessary to separate and purify nucleic acids or proteins, even cells or bacteria, and the magnetic bead method is one of the most commonly used separation and purification methods. However, in the process of sucking the magnetic bead suspension, the magnetic beads will gradually settle under the action of gravity, which makes the magnetic bead suspension gradually layer, and finally makes the concentration of the magnetic bead sucked into each sample uneven, which is very large. Affect the experimental results. [0003] In today's automated liquid workstations, the following equipment or methods are used to maintain the uniform distribution of magnetic bead suspensions: 1) Propeller stirrer or ultrasonic stirrer is used. The disadvantage of this ty...

AI Technical Summary

Problems solved by technology

However, in the process of absorbing the magnetic bead suspension, the magnetic beads tend to gradually aggregate and settle under the action of gravity, which makes the magnetic bead suspension gradually stratified, and finally makes the concentration of the magnetic beads absorbed into each sample uneven and large. affect the experimental results

[0003] In today's automated liquid workstations, the following equipment or methods are mainly used to maintain the uniform distribution of the magnetic bead suspension: 1) Using a propeller stirrer or an ultrasonic stirrer, the disadvantage of this type of technology is that it is easy to make the magnetic bead suspension splash out of the container, Cause pollution and loss of magnetic beads, and easily damage the sample; 2) Use the method of blowing and mixing with the pipette tip to maintain the uniform distribution of the magnetic bead suspension. The disadvantage of this method is that it cannot meet the requirements of mixing and absorbing magnetic beads. At the same time, it is necessary to suspend the mixing when absorbing the magnetic beads, and the magnetic beads have begun to precipitate, which will finally affect the experimental results; 3) Use a magnetic stirrer or a magnetic field stirrer. The disadvantage of using this method is that some magnetic beads will Adsorbed to the magnet block, resulting in uneven distribution of magnetic beads in the suspension, and loss of magnetic beads when the magnet block is taken out

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