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A method for producing palmitoleic acid by utilizing microalgae of the class Euophthora

A technology of eustoma and palmitoleic acid, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high cost, low purity of palmitoleic acid, and unsuitability for large-scale production, etc. achieve simple results

Active Publication Date: 2021-03-19
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although both supercritical extraction and molecular distillation can be applied to the extraction and concentration of palmitoleic acid from microalgae, they are expensive and not suitable for large-scale production, and the purity of palmitoleic acid is not high

Method used

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  • A method for producing palmitoleic acid by utilizing microalgae of the class Euophthora
  • A method for producing palmitoleic acid by utilizing microalgae of the class Euophthora
  • A method for producing palmitoleic acid by utilizing microalgae of the class Euophthora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 The optimal initial nitrogen concentration of E.cf.polyphem highly accumulating palmitoleic acid

[0040] Experimental material: E.cf.polyphem (E.cf.polyphem) (expanded and cultivated)

[0041] Medium preparation: mBG-11 liquid medium, 1.5g / L NaNO 3 , 0.04g / L K 2 HPO 4 , 0.075g / LMgSO 4 ·7H 2 O, 0.036g / L CaCl 2 2H 2 O, 0.006g / L citric acid, 0.006g / L FeCl 3 ·6H 2 O, 0.001g / LEDTA, 0.02g / L Na 2 CO 3 , 2.860g / L H 3 BO 3 , 1.810g / L MnCl 2 4H 2 O, 0.391g / L Na 2 MoO 4 2H 2 O, 0.079g / L CuSO 4 ·5H 2 O, 0.220g / L ZnSO 4 ·7H 2 O, 1L deionized water, sterilized at 121°C for 20min. where NaNO 3 As a nitrogen source, 4 different initial concentrations were set, namely 18mM (1.5g), 9mM (0.75g), 3mM (0.25g) and 1mM (0.08g).

[0042] experiment method:

[0043] The strains of E.cf.polyphem that had been expanded were divided according to the OD 750 = 0.60 ± 0.02 initial inoculation density into the optical columnar glass bioreactor (diameter × height = 3...

Embodiment 2

[0047] Phase changes in the growth and palmitoleic acid accumulation of E.cf.polyphem (E.cf.polyphem) under nitrogen stress conditions (1mM) in Example 2

[0048] Experimental material: Euophthalmos bordetii-like (expansion and cultivation have been carried out)

[0049] Medium preparation: mBG-11 liquid medium, 0.08g / L NaNO 3 , 0.04g / L K 2 HPO 4 , 0.075g / LMgSO 4 ·7H 2 O, 0.036g / L CaCl 2 2H 2 O, 0.006g / L citric acid, 0.006g / L FeCl 3 ·6H 2 O, 0.001g / LEDTA, 0.02g / L Na 2 CO 3 , 2.860g / L H 3 BO 3 , 1.810g / L MnCl 2 4H 2 O, 0.391g / L Na 2 MoO 4 2H 2 O, 0.079g / L CuSO 4 ·5H 2 O, 0.220g / L ZnSO 4 ·7H 2 O, 1L deionized water, sterilized at 121°C for 20min.

[0050] experiment method:

[0051] The euopteris strains that have been expanded were divided according to the OD 750=0.60±0.02 initial inoculation density was inserted into mBG-11 liquid medium with an initial nitrogen concentration of 1 mM, and cultured in a bright columnar glass bioreactor with diameter×heigh...

Embodiment 3

[0053] The preparation of palmitoleic acid in the embodiment 3 classes E.cf.polyphem (E.cf.polyphem)

[0054] Experimental material: Freeze-dried algae powder of E.cf.polyphem under the culture condition of 1mM nitrogen concentration

[0055] experiment method:

[0056] (1) Oil extraction: take freeze-dried algae powder and 95% ethanol and mix according to the ratio of m / v=1:30, put it in an ultrasonic cleaning machine and continue ultrasonic treatment for 2.5 hours. After ultrasonic pretreatment, the flask containing the algae solution was placed in a constant temperature water bath at 50° C., and extracted for 4 hours with continuous stirring (200 rpm) by an electric stirrer. After the extraction, centrifuge at 3500rpm for 5min, collect the supernatant, and continue to repeat the above extraction steps for 3 to 4 times for algae. After combining the supernatants, use a rotary evaporator (50° C.) to recover ethanol to obtain total lipids. Detected by gas chromatography, as...

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Abstract

The invention discloses a method for producing palmitoleic acid from Eustigmatophyceae microalgae, and relates to thel fields of microalga biological resources and the biotechnology. The preparation method comprises the following steps: total fat extraction, and concentrating of palmitoleic acid. The method for producing palmitoleic acid from E.cf. polyphem is green and safe, and the source of theraw material s not restricted by the geography or season, can be continuously harvested all through the year, and can meet a huge raw material gap. The preparation method for efficiently concentrating the palmitoleic acid, provided by the invention, has the advantages of simplicity in operation, high efficiency, low cost and high palmitoleic acid purity, is suitable for large-scale production, and lays a foundation for the commercial development of microalga-derived palmitoleic acid products.

Description

technical field [0001] The invention relates to the field of microalgae biological resources and biotechnology, in particular to a method for producing palmitoleic acid by utilizing microalgae of the class Eutectica. Background technique [0002] Palmitoleic acid (C16:1ω7, palmitoleic acid) is a monounsaturated fatty acid consisting of sixteen carbon atoms and containing an ω-7 unsaturated double bond. The structural formula is shown in Formula 1. In recent years, a large number of biological studies have found that palmitoleic acid plays an important role in the balance of glucose and lipid metabolism in organisms, and this fatty acid has a positive effect on some chronic diseases, such as obesity, diabetes, dyslipidemia, inflammation and atherosclerosis. Has a certain therapeutic effect. Therefore, its medical and health care value has gradually attracted people's attention. [0003] my country's population structure has entered an aging population, and the population ba...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12R1/89
CPCC12P7/6409
Inventor 张成武王飞飞高保燕黄罗冬
Owner JINAN UNIVERSITY
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