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Method for improving image signal-to-noise ratio by using water-soluble light absorbent

A light absorber and water-soluble technology, applied in the field of using water-soluble light absorbers to improve the image signal-to-noise ratio, can solve the problems of slow imaging speed, poor maintenance, unfavorable shape, etc.

Active Publication Date: 2018-12-18
HUST SUZHOU INST FOR BRAINMATICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since SBB is a fat-soluble dye that needs to be dissolved in alcohol, it will cause a certain degree of deformation of the tissue after it penetrates into the biological tissue, which is not conducive to the maintenance of the shape
[0007] To sum up, the current methods of suppressing tissue background fluorescence to improve image signal-to-noise ratio have the problems of slow imaging speed, incomplete suppression of background fluorescence, or poor maintenance of tissue morphology.

Method used

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  • Method for improving image signal-to-noise ratio by using water-soluble light absorbent
  • Method for improving image signal-to-noise ratio by using water-soluble light absorbent
  • Method for improving image signal-to-noise ratio by using water-soluble light absorbent

Examples

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Effect test

example 1

[0047] Example 1 Fluorescent protein labeled mouse brain tissue samples

[0048] Step 1: Inject a mixture of 10% urethane and 2% chloral hydrate into the abdominal cavity of two-month-old Thy1-GFP male mice for anesthesia, perfusion to take the brain, fixation with PFA for 24 hours, and rinse with PBS for 24 hours;

[0049] Step two, slice the whole mouse brain on a vibrating microtome for 200-500um;

[0050] Step 3: Dissolve a certain concentration of water-soluble light absorber in PBS, and put the sliced ​​brain slices into PBS-water-soluble light absorber solution (the mass concentration of water-soluble light absorber is 0.1%-5%) and penetrate 0.1-10h, use Keyamine as water-soluble light absorber TM Black SP-IJ Liquid;

[0051] Step 4: Mount the infiltrated brain slice and image it on a fluorescence microscope. A wide-field fluorescence microscope can be used;

[0052] by Figure 1 ~ Figure 4 It can be seen from the content shown that after the light absorber-PBS solution is used ...

example 2

[0053] Example 2 Dye-labeled mouse brain tissue samples

[0054] Step 1: Inject DyLight 488 into the tail vein of two-month-old C57 male mice. After 20 minutes, they were injected with a mixture of 10% urethane and 2% chloral hydrate for anesthesia. The brain was taken out by perfusion, fixed with PFA for 24 hours, and rinsed with PBS for 24 hours;

[0055] Step two, slice the whole mouse brain for 200-500μm on a Leica commercial vibrating microtome;

[0056] Step 3: Dissolve a certain concentration of water-soluble light absorber in PBS, and put the sliced ​​brain slices into PBS-water-soluble light absorber solution (the mass concentration of water-soluble light absorber is 0.1%-5%) and penetrate 0.1-10h, water-soluble light absorber selected from Keyamine TM Black SP-IJ Liquid;

[0057] Step 4: Mount the infiltrated brain slice and image it on a fluorescence microscope;

[0058] by Figure 5 ~ Figure 7 From the content shown, it can be seen that before and after using the light abs...

example 3

[0059] Example 3 Dye-labeled mouse kidney samples

[0060] Step 1: Inject DyLight 488 into the tail vein of two-month-old C57 male mice. After 20 minutes, they were injected with a mixture of 10% urethane and 2% chloral hydrate for anesthesia. Kidneys were taken by perfusion, fixed with PFA for 24 hours, and rinsed with PBS for 24 hours;

[0061] Step 2: Embed the mouse kidney with agarose and slice it 200-500μm on the Lycra commercial vibrating microtome;

[0062] Step 3: Dissolve a certain concentration of water-soluble light absorber in PBS, and put the cut kidney tissue into PBS-water-soluble light absorber solution (the mass concentration of water-soluble light absorber is 0.1%-5%) to penetrate 0.1-10h, water-soluble light absorber selected from Keyamine TM Black SP-IJ Liquid;

[0063] Step 4: Mount the infiltrated kidney tissue and image it on a fluorescence microscope;

[0064] by Picture 8 From the content shown, it can be seen that before and after using the light absorber, t...

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Abstract

The invention discloses a method for improving an image signal-to-noise ratio by using a water-soluble light absorbent. The method includes the steps that biological tissues are pretreated, and a water-soluble light absorbent-PBS mixed solution is prepared; permo-treatment is carried out on the pretreated tissues in the water-soluble light absorbent-PBS mixed solution; and the permeated tissues are imaged on an imaging system. The invention provides the method for inhibiting tissue background fluorescence based on the water-soluble light absorbent and improving the image signal-to-noise ratio,the biological tissues do not need to be dehydrated during use, simple operation is achieved, tissue deformation cannot be caused, and universality is good.

Description

Technical field [0001] The invention relates to the technical field of biological optical imaging, in particular to a method for improving the signal-to-noise ratio of an image by using a water-soluble light absorber. Background technique [0002] Biological optical imaging (Optical Imaging) refers to a method that uses optical detection means combined with optical detection molecules to image cells or tissues and even organisms to obtain biological information. If bio-optical imaging is limited to visible light and near-infrared light, according to different detection methods, bio-optical imaging can be divided into fluorescence imaging, bioluminescence imaging, photoacoustic imaging, optical tomography, etc. [0003] Bio-optical imaging can be used to obtain structural information of biological tissues such as brain, heart, liver, kidney, and pancreas. For example, in the field of neuroscience, we can obtain the fine morphology of different types of neurons and their precise loc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N1/28
CPCG01N1/28G01N21/64
Inventor 龚辉李向宁周灿罗婷
Owner HUST SUZHOU INST FOR BRAINMATICS