A method for improving image signal-to-noise ratio by using water-soluble light absorber
A light absorber and water-soluble technology, which is applied in the field of using water-soluble light absorbers to improve the image signal-to-noise ratio, can solve the problems of unfavorable morphology, poor maintenance, incomplete background fluorescence suppression, tissue morphology, etc.
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example 1
[0047] Example 1 Fluorescent protein labeling of mouse brain tissue samples
[0048] Step 1: Two-month-old Thy1-GFP male mice were anesthetized by intraperitoneal injection of a mixture of 10% urethane and 2% chloral hydrate, and the brain was taken out by perfusion, fixed with PFA for 24 hours, and rinsed with PBS for 24 hours;
[0049] Step 2, slice the mouse whole brain on a vibrating microtome at 200-500um;
[0050] Step 3, dissolve a certain concentration of water-soluble light absorber in PBS, put the cut brain slice into PBS-water-soluble light absorber solution (the mass concentration of water-soluble light absorber is 0.1%-5%) infiltration 0.1-10h, water-soluble light absorber chooses Keyamine TM Black SP-IJ Liquid;
[0051] Step 4, seal the infiltrated brain slices, and image them on a fluorescence microscope, a wide-field fluorescence microscope can be used;
[0052] pass Figure 1 ~ Figure 4 From the content shown, it can be seen that after using the light abso...
example 2
[0053] Example 2 dye-labeled mouse brain tissue samples
[0054]Step 1: Two-month-old C57 male mice were injected with DyLight 488 into the tail vein, 20 minutes later, intraperitoneally injected with a mixture of 10% urethane and 2% chloral hydrate for anesthesia, the brain was perfused, fixed with PFA for 24 hours, and rinsed with PBS for 24 hours;
[0055] Step 2: Slice the whole brain of the mouse at 200-500 μm on a commercial Leica vibrating microtome;
[0056] Step 3, dissolve a certain concentration of water-soluble light absorber in PBS, put the cut brain slice into PBS-water-soluble light absorber solution (the mass concentration of water-soluble light absorber is 0.1%-5%) infiltration 0.1-10h, the water-soluble light absorber is selected from Keyamine TM Black SP-IJ Liquid;
[0057] Step 4: Seal the infiltrated brain slices and image them on a fluorescent microscope;
[0058] pass Figure 5 ~ Figure 7 It can be seen from the shown content that the position and sh...
example 3
[0059] Example 3 dye-labeled mouse kidney sample
[0060] Step 1: Two-month-old C57 male mice were injected with DyLight 488 into the tail vein, 20 minutes later, intraperitoneally injected with a mixture of 10% urethane and 2% chloral hydrate for anesthesia, and the kidneys were collected by perfusion, fixed with PFA for 24 hours, and rinsed with PBS for 24 hours;
[0061] Step 2: After embedding the mouse kidney in agarose, slice it into 200-500 μm slices on a Lycra commercial vibrating microtome;
[0062] Step 3, dissolving a certain concentration of water-soluble light absorber in PBS, and infiltrating the cut kidney tissue into PBS-water-soluble light absorber solution (the mass concentration of water-soluble light absorber is 0.1%-5%) 0.1-10h, the water-soluble light absorber is selected from Keyamine TM Black SP-IJ Liquid;
[0063] Step 4: Seal the infiltrated kidney tissue and image it on a fluorescence microscope;
[0064] pass Figure 8 From the contents shown, i...
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