Marker related to malignant degree of oral squamous cell carcinoma and application thereof

A squamous cell carcinoma, malignant technology, applied in the field of biomedicine, can solve the problems of reduced quality of life, facial appearance, etc.

Inactive Publication Date: 2018-12-21
SUINING CENT HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, after comprehensive treatment such as surgery, radiotherapy and chemotherapy, the facial appearance of advanced patients is severely damaged, and the quality of life is significantly reduced.

Method used

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  • Marker related to malignant degree of oral squamous cell carcinoma and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Screening of molecular markers associated with malignancy of oral squamous cell carcinoma

[0041] 1. Clinical research objects:

[0042] Selected 15 cases of oral squamous cell carcinoma resected by maxillofacial surgery in the hospital (all patients were not treated with preoperative radiotherapy and chemotherapy), including 6 cases of well-differentiated (grade III, G3), moderately differentiated (grade II, G2) ) in 4 patients and 5 patients with poorly differentiated (grade I, G1) patients (diagnosed by two experienced pathologists who determined the pathological grade of all cancer tissues).

[0043] 2. Tissue RNA extraction

[0044] (1) Tissue grinding: First, put a small amount of liquid nitrogen into a mortar, put about 100 mg of the prepared tissue into it, take out the tissue stored in the -80 degree ultra-low temperature refrigerator, put it in the mortar and add a small amount of liquid Nitrogen, add liquid nitrogen while grinding to prevent liqu...

Embodiment 2

[0071]Example 2 QPCR verifies the relationship between candidate genes and the malignancy of oral squamous cell carcinoma

[0072] Based on the results of the previous high-throughput sequencing, according to the size of the P value, we selected the SIX4 gene for verification.

[0073] 1. Research objects:

[0074] Selected 90 cases of oral squamous cell carcinoma resected by maxillofacial surgery in the hospital's department of stomatology (all patients were not treated with preoperative radiotherapy and chemotherapy), including 30 patients with high differentiation (G3), 30 patients with moderate differentiation (G2), and 30 patients with low differentiation (G2). There were 30 differentiated (G1) patients (diagnosed by two experienced pathologists who determined the pathological grade of all cancer tissues).

[0075] 2. RNA extraction and detection

[0076] Follow the method of Example 1.

[0077] 3. Reverse transcription

[0078] cDNA was synthesized by reverse transcr...

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Abstract

The invention discloses an SIX4 gene used as a molecular marker for assessing the malignant degree of oral squamous cell carcinoma. High-throughput sequencing and QPCR studies show that the content ofthe SIX4 gene in high differentiated oral squamous cell carcinoma is lower than that in low differentiated oral squamous cell carcinoma. According to the research results, the invention can also develop a kit for assessing the malignant degree of oral squamous cell carcinoma, which has a remarkable detection effect and can be widely popularized and applied in clinic.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular, the present invention relates to a marker related to the malignancy of oral squamous cell carcinoma and its application, more particularly, to the application of SIX4 as a molecular marker of the malignancy of oral squamous cell carcinoma. Background technique [0002] Oral squamous cell carcinoma is one of the ten most common cancers in the world, and it is the most common oral tumor, accounting for more than 90%. Oral squamous cell carcinoma not only endangers the patient's life, but also affects its appearance and appearance, as well as the patient's quality of life such as chewing, speech, and swallowing. [0003] The occurrence of oral squamous cell carcinoma is related to many factors, and is often the result of the combined effect of many factors. In clinical work, oral cancer is defined as including gum cancer, tongue cancer, soft and hard cancer, maxillary and mandibula...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11G01N33/68G01N33/574
CPCC12Q1/6886C12Q2600/118C12Q2600/156G01N33/57407G01N33/68G01N2333/46
Inventor 杨森郭丽娟
Owner SUINING CENT HOSPITAL
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