Preparation method, application and preparation primer of influenza virus HA fusion peptide and helix A protein

A technology of influenza virus and fusion peptide, applied in the direction of virus peptide, fusion peptide, chemical instruments and methods, etc., to achieve good immunogenicity effect

Inactive Publication Date: 2018-12-25
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, whether the fusion peptide of HA and the helix A region can be used

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  • Preparation method, application and preparation primer of influenza virus HA fusion peptide and helix A protein
  • Preparation method, application and preparation primer of influenza virus HA fusion peptide and helix A protein
  • Preparation method, application and preparation primer of influenza virus HA fusion peptide and helix A protein

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[0029] Example

[0030]1. Design and amplify primers containing pGEX-6p-1 linearized vector and influenza virus HA fusion peptide and helix A gene fragment: The upstream primer for amplifying pGEX-6p-1 linearized vector is located at positions 1022-1047 of pGEX-6p-1 plasmid and with an extra TAA base at the 5' end; the downstream primer for amplifying the pGEX-6p-1 linearized vector is located at positions 916-941 of the pGEX-6p-1 plasmid; and with an extra CAT base at the 5' end. Amplify the HA fusion peptide of influenza virus and the upstream primer of helix A gene. ATG GGA AGC is a strong promoter including 17 bases of HA fusion peptide and helix A gene, and 13 bases with pGEX-6p-1 at the 5' end Additional bases for reverse complementation of the primers downstream of the linearized vector; amplification of the influenza virus HA fusion peptide and the helix A gene downstream primers include the HA fusion peptide and the helix A gene stop codon TAA and its first 15 bases, ...

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Abstract

The invention discloses a preparation method, application and preparation primer of an influenza virus HA fusion peptide and helix A protein expression vector. The principle and key technique herein are to scientifically design and amplify a pGEX-6P-1 linear vector and influenza virus HA fusion peptide-helix A gene fragment; commercial recombinant enzyme Exnase TM II is subjected directly to in-vitro quick recombinant cloning of HA fusion peptide-helix A without enzyme-cut and link-up connection; Escherichia coli is converted and expressed under IPTG induction, and fusion expression of influenza virus HA fusion peptide-helix A protein with GST is achieved; the purified HA fusion protein-helix A fusion protein is attained.

Description

technical field [0001] The invention relates to a preparation method and application of influenza virus HA fusion peptide and helix A protein. The invention can directly provide HA fusion peptide and helix A fusion protein as immunogens to obtain anti-HA fusion peptide and helix A polyclonal antibody. The invention will lay a foundation and provide materials for further exploring whether the fusion peptide of HA and the helix-A region can be used as an effective epitope to stimulate the body to produce neutralizing antibodies, and to evaluate the broad-spectrum influenza neutralizing subunit vaccine. Background technique [0002] The use of vaccines and antiviral drugs has made positive contributions to the prevention and control of influenza viruses. However, due to the continuous variation of influenza virus antigens and the continuous emergence of recombinant viruses, human influenza pandemics and avian influenza outbreaks often occur, seriously affecting human health an...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12N15/70C12N15/62C07K19/00
CPCC07K14/005C07K2319/00C12N15/70C12N2760/16122
Inventor 叶建强李占萍邵红霞秦爱建万志敏
Owner YANGZHOU UNIV
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