Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of circRNA marker and application thereof for diagnosis of breast cancer

A breast cancer and forward primer technology, applied in the field of biomedicine, can solve problems such as circRNA in whole blood of breast cancer that have not yet been seen

Active Publication Date: 2021-08-03
SHANDONG UNIV QILU HOSPITAL
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the inventor has not seen the report of circRNA in whole blood of breast cancer as a marker

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of circRNA marker and application thereof for diagnosis of breast cancer
  • A kind of circRNA marker and application thereof for diagnosis of breast cancer
  • A kind of circRNA marker and application thereof for diagnosis of breast cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A detection kit for diagnosing breast cancer, the detection kit, detection system includes a reverse transcription reaction system and qPCR reaction system, the detection kit includes reagents for preparing the reverse transcription reaction system and for Prepare reagents for qPCR reaction system.

[0049] The reagents used to prepare the reverse transcription reaction system include at least reverse transcription buffer, dNTP mixed solution, RNase protein inhibitor, reverse transcriptase solution and the like.

[0050] The reagents used to prepare the qPCR reaction system include at least forward and reverse primers for one or more combinations of circ_0000745, circ_0001531, and circ_0001640, as well as forward and reverse primers for internal reference genes, SYBR Green mixed solution, nuclease-free pure water, etc. See Table 1 for the relevant primer sequences.

Embodiment 2

[0052] (1) Clinical samples

[0053] From June 27, 2016 to August 30, 2017, peripheral blood from 68 breast cancer patients and 13 healthy controls were recruited at Qilu Hospital of Shandong University. All patients and controls were Han females.

[0054] Fasting venous whole blood (2ml) was collected and untreated whole blood samples were immediately stored in liquid nitrogen until further analysis. All breast cancer blood samples were collected before surgery or any treatment. Control samples were collected from healthy females with no past or present history of malignancy or inflammation. All participants obtained written informed consent. This study was approved by the Ethics Committee of Qilu Hospital of Shandong University.

[0055] (2) RNA extraction

[0056] Take 250ul of whole blood and transfer it to a 1.5ml centrifuge tube, add 750μl of TRIzol LS Reagent and 20μl of glacial acetic acid, shake the tube vigorously by hand until mixed. After homogenization, the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present disclosure relates to a circRNA marker for the diagnosis of breast cancer and its circRNA application. Compared with the healthy control group, three circRNAs (hsa_circ_0000745, hsa_circ_0001531, hsa_circ_0001640) were found to be up-regulated in whole blood of breast cancer. ROC curve analysis shows that it can better distinguish breast cancer patients from healthy people. The areas under the curves of the three are 0.7998, 0.8258, and 0.7161, the sensitivities are 48.53%, 92.65%, 73.53%, and the specificities are 100%. , 69.23%, 69.23%.

Description

technical field [0001] The disclosure belongs to the field of biomedicine, and in particular relates to a circRNA marker for breast cancer diagnosis and its application. Background technique [0002] The statements herein merely provide background information related to the present disclosure and may not necessarily constitute prior art. [0003] Breast cancer is a malignant tumor that occurs in the glandular epithelial tissue of the mammary gland, which seriously threatens women's health. The occurrence and development of breast cancer are related to the abnormal expression of many tumor markers. At present, there are many breast cancer tumor markers: progesterone receptor (PR), vascular endothelial growth factor (VEGF), estrogen receptor (ER ), CD44, p53, etc. But its real clinical application is very limited. Therefore, it is very important and urgent to further develop new non-invasive biomarkers for detecting breast cancer with higher sensitivity and specificity. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 张凯马榕王亚文朱江陈旭
Owner SHANDONG UNIV QILU HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com