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Method of determining micro calcium ions through dispersive liquid-liquid microextraction-Android mobile phone colorimetry

A technology of dispersion liquid and Android mobile phone, applied in the field of cation detection, can solve the problems of long detection time, poor reproducibility and selectivity, complicated operation, etc., and achieve the effect of easy on-site deployment, low instrument cost and simple operation

Active Publication Date: 2018-12-28
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among these conventional methods, titration and photometry have low sensitivity, poor reproducibility and selectivity, and spectroscopic instruments have defects such as high cost, long detection time, complicated operation, and inability to perform real-time on-site detection.

Method used

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  • Method of determining micro calcium ions through dispersive liquid-liquid microextraction-Android mobile phone colorimetry
  • Method of determining micro calcium ions through dispersive liquid-liquid microextraction-Android mobile phone colorimetry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, the detection of calcium content in urban tap water

[0026] (1) Sample configuration: accurately pipette 1.0mL tap water sample, add to a 50mL volumetric flask, dilute to 50mL; accurately pipette 1.0mL of this solution into a centrifuge tube, add 0.3mL 1.25×10 -3 mol / L GBHA solution, 1.7mL ethanol, 3mL borax-sodium hydroxide buffer solution with a pH of 12.8, shake well, let stand for 3min, dilute to 10mL with ultrapure water, then add 1.4mL 5×10 -4 mol / L tetradecyl dimethyl benzyl ammonium chloride aqueous solution, 300 μL of a mixture of chloroform and dichloromethane (v / v=1 / 3), shake vigorously for 3 minutes, and then centrifuge for 12 minutes (3000 rpm / min); the colored matter is enriched at the bottom of the centrifuge tube;

[0027] (2) Preparation of blank control solution: mix the reagent blank (other reagents except tap water) under the same conditions to obtain a blank control solution, and follow the same steps for processing;

[0028] (3) ...

Embodiment 2

[0033] Embodiment 2, the detection of calcium in Nongfu mountain spring water

[0034] (1) Sample configuration: Accurately pipette 0.5mL of Nongfu mountain spring water into a 10mL centrifuge tube, add 0.4mL, 1.25×10 -3mol / L GBHA solution, 1.6mL ethanol, 3mL borax-sodium hydroxide buffer solution with pH=12.8, shake well, let stand for 3min, and dilute to 10.0mL. Then add 1.4mL5×10 -4 mol / L tetradecyl dimethyl benzyl ammonium chloride aqueous solution, 300 μL of a mixture of chloroform and dichloromethane (v / v=1 / 3), shake vigorously for 3 minutes, and then centrifuge for 12 minutes (3000 rpm / min), the colored matter is enriched at the bottom of the centrifuge tube;

[0035] (2) Preparation of blank control solution: mix the reagent blank (other reagents except Nongfu Spring) under the same conditions to obtain a blank control solution, and follow the same steps;

[0036] (3) Y G Calculation: In the mobile phone test box, use the smart phone to obtain the RGB value of th...

Embodiment 3

[0041] Example 3. Detection of Calcium Content in National Standard Material Water Quality Calcium (Standard Sample) (GSB07-1192-2000)

[0042] (1) Sample configuration: accurately pipette 0.1mL of aqueous calcium solution into a 10mL centrifuge tube, add 0.5mL, 1.25×10 -3 mol / L GBHA solution, 1.5 mL of ethanol, 3 mL of borax-sodium hydroxide buffer solution with pH=12.8, shake well, let stand for 3 minutes, and dilute to 10 mL. Then add 1.4mL5×10 -4 mol / L tetradecyl dimethyl benzyl ammonium chloride aqueous solution, 300 μL of a mixture of chloroform and dichloromethane (v / v=1 / 3), then centrifuge for 12 min (3000 rpm), The colored matter is concentrated at the bottom of the centrifuge tube.

[0043] (2) Preparation of blank control solution: mix the reagent blank (other reagents except the marble digestion solution sample) under the same conditions to obtain a blank control solution, which is operated as above;

[0044] (3) Y G Calculation: In the mobile phone test box, ...

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Abstract

The invention discloses a method of determining micro calcium ions through dispersive liquid-liquid microextraction-Android mobile phone colorimetry. The method comprises the steps of taking glyoxalbishydroxyanil as a developer to react with the Ca ions in a borax-sodium hydroxide buffer solution with a pH (potential of hydrogen) of 12.8 to generate an orange hydrophobic complex, using tetradecylbenzyl dimethyl ammonium chloride as a surfactant, enriching and extracting the complex into a mixed extraction agent of trichloromethane and dichloromethane through a DLLME (dispersive liquid-liquidmicroextraction) technology, obtaining an RGB (red, green and blue) color intensity value of the solution through Android mobile phone software Color Grab, determining a quantitative relationship between color intensity of a sample and a Ca<2+> concentration, and obtaining a content of the calcium ions in the solution to be determined. The method is high in detection sensitivity and selectivity; amobile phone testing case is simple to operate and easy to carry; and the method can be deployed on site and is applicable to quick determination of ppb (part per billion) level calcium in water.

Description

technical field [0001] The invention relates to a method for detecting trace calcium ions, in particular to a method for detecting trace calcium ions by dispersion liquid-liquid microextraction-android mobile phone colorimetry, which belongs to the field of cation detection. Background technique [0002] Calcium is one of the most important elements in the human body and widely exists in environmental water bodies. At present, there are many methods for the determination of calcium, such as complexometric titration, automatic potentiometric titration, spectrophotometry, atomic absorption spectrometry, atomic emission spectrometry, etc. Among these conventional methods, titration and photometry have low sensitivity, poor reproducibility and selectivity, and spectroscopic instruments have defects such as high cost, long detection time, complicated operation, and inability to perform real-time on-site detection. Therefore, it is of great significance to develop a method for th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/25G01N1/34
CPCG01N1/34G01N21/25
Inventor 彭波周娟娟徐佳美黎凯樊祢祢
Owner NORTHWEST NORMAL UNIVERSITY
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