Adult stem cell lyophilized powder preparation method and application thereof to cosmetics

An adult stem cell and freeze-dried powder technology, applied in the biological field, can solve the problems of lack of universal applicability, immune rejection, and high cost, and achieve the effects of improving skin condition, resisting and cell aging, and shortening growth cycle.

Inactive Publication Date: 2019-01-04
广州苿莱生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are some so-called stem cell injections in the beauty industry on the market. The main source of stem cells is extracted from the embryos produced. If the embryonic stem cells are directly injected without matching, it is easy to cause immune rejection, which costs a lot and is expensive. Need to be customized according to individual circumstances, there is no universal applicability

Method used

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  • Adult stem cell lyophilized powder preparation method and application thereof to cosmetics
  • Adult stem cell lyophilized powder preparation method and application thereof to cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Prepare serum-free medium

[0023] Take alpha-MEM medium, add serum substitute, L-glutamine, ribonucleoside, deoxyribonucleoside, fibroblast growth factor and transforming growth factor-β, wherein the amount of serum substitute is volume fraction 8%, the concentration of L-glutamine in the serum-free medium is 280ng / ml, the concentration of the ribonucleoside is 10ng / ml, and the concentration of the deoxyribonucleoside is 10ng / ml, so The concentration of the fibroblast growth factor was 15 ng / ml, and the concentration of the transforming growth factor-β was 15 ng / ml to obtain a culture solution.

[0024] Culture adult stem cells and prepare lyophilized powder

[0025] Take human adipose tissue, wash it with phosphate for 5 times, add enzymes to digest and centrifuge, wash the obtained cells with phosphate buffer, mix the cells and culture medium, add them to T175 cell culture flasks, and store them at 37°C with an oxygen concentration of 2%. , cultured under the condi...

Embodiment 2

[0027] Prepare serum-free medium

[0028] Take alpha-MEM medium, add serum substitute, L-glutamine, ribonucleoside, deoxyribonucleoside, fibroblast growth factor and transforming growth factor-β, wherein the amount of serum substitute is volume fraction 10%, the concentration of L-glutamine in the serum-free medium is 300ng / ml, the concentration of the ribonucleoside is 20ng / ml, and the concentration of the deoxyribonucleoside is 20ng / ml, so The concentration of the fibroblast growth factor is 25 ng / ml, and the concentration of the transforming growth factor-β is 25 ng / ml to obtain a culture solution.

[0029] Culture adult stem cells and prepare lyophilized powder

[0030] Extract blood stem cells by using a fully automatic blood cell separator, mix the cells with the culture medium, add them to a T175 cell culture flask, and culture them at 37°C, with an oxygen concentration of 3% and a carbon dioxide concentration of 5% to a level of more than 85% of the cells Subculture ...

Embodiment 3

[0032] Prepare serum-free medium

[0033] Take alpha-MEM medium, add serum replacement, L-glutamine, ribonucleoside, deoxyribonucleoside, fibroblast growth factor, transforming growth factor-β, vitamin B5, vitamin B12, vitamin C and vitamin E, wherein the addition amount of the serum substitute is 10% by volume, the concentration of L-glutamine in the serum-free medium is 280ng / ml, and the concentration of the ribonucleoside is 10ng / ml, the The concentration of deoxyribonucleosides is 10ng / ml, the concentration of the fibroblast growth factor is 15ng / ml, the concentration of the transforming growth factor-beta is 15ng / ml, and the concentration of the vitamin B5 is 3ng / ml, The concentration of vitamin B12 was 0.01 ng / ml, the concentration of vitamin C was 30 ng / ml, and the concentration of vitamin E was 0.3 ng / ml to obtain a culture solution.

[0034] Culture adult stem cells and prepare lyophilized powder

[0035] Take human adipose tissue, wash it with phosphate for 5 times, ...

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Abstract

The invention provides an adult stem cell lyophilized powder preparation method. Adult stem cells extracted from human fat or blood serve as raw materials to be cultured in a serum-free medium. The serum-free medium is formed by adding a serum substitute, L-glutamine, ribonucleotide, deoxyribonucleoside, fibroblast growth factors and transforming growth factor-beta into a basal medium. The serum-free medium is high in safety and less prone to contamination in a cell culture process, an adult stem cell growth period can be sharply shortened, the adult stem cell proliferation speed is increased,growth factor secretion of the adult stem cells is promoted, and the obtained adult stem cell lyophilized powder is high in purity and bioactivity and effective in damaged cell repair, promotion of skin cell growth and proliferation, elimination of skin wrinkles and delaying and resisting of senescence.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a preparation method of adult stem cell freeze-dried powder and its application in anti-aging cosmetics. Background technique [0002] Beauty is the nature of human beings, and skin aging is the natural enemy of beauty lovers. Skin aging refers to the aging damage of skin function, which makes the skin's ability to protect the body and adjust its ability to decline. The aging of the skin is characterized by small wrinkles, decreased elasticity, and sagging of the skin. Skin aging is divided into endogenous aging and exogenous aging. Endogenous aging is aging controlled by genetic factors and naturally ages with age; exogenous aging is aging caused by environmental factors, including sunlight. , mechanical action, pollution, excessive skin beauty, etc. The growth period of skin tissue generally ends around the age of 25, after which growth and aging coexist, the elastic fibers...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): A61K8/99A61K8/98A61K8/64A61K8/02A61Q19/08
CPCA61K8/022A61K8/64A61K8/981A61K8/983A61K8/99A61K2800/805A61Q19/08
Inventor程文海
Owner广州苿莱生物科技有限公司