Unlock instant, AI-driven research and patent intelligence for your innovation.

System for transforming skin fibroblast cells into artificial intervertebral disc and using method thereof

A technology of fibroblasts and intervertebral discs, applied in the field of skin fibroblasts into artificial intervertebral discs, can solve the problems of limited application prospects, difficulty in ensuring the quantity and quality of cartilage or nucleus pulposus cells, and achieve the regulation of intervertebral disc degeneration and improvement Intervertebral disc signal and the effect of maintaining intervertebral space height

Pending Publication Date: 2019-01-04
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of the described method is that the cells for this use need to be harvested from adjacent healthy intervertebral discs or from other donors of the same species
Secondly, the primary cartilage or nucleus pulposus cells age obviously after being cultured in vitro for several generations, and it is difficult to ensure the quantity and quality of cartilage or nucleus pulposus cells reinjected into the intervertebral space
The application prospect of this method is obviously limited

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • System for transforming skin fibroblast cells into artificial intervertebral disc and using method thereof
  • System for transforming skin fibroblast cells into artificial intervertebral disc and using method thereof
  • System for transforming skin fibroblast cells into artificial intervertebral disc and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Cut the skin tissue into pieces (0.5cm*0.5cm), soak in 75% alcohol for 30 minutes, then put it in 0.25% dispase solution at 4°C overnight; take out the skin tissue the next day, and tear off the epidermal tissue , then cut the tissue into small pieces, and then put it into 0.25% collagenase for digestion at 37°C for 4 hours; after 4 hours, observe whether the tissue is digested cleanly, and if it is not digested cleanly, the digestion time can be extended appropriately; after digestion is completed, filter (single-cell strainer) ), centrifuge (5min, 1500rpm, 37°C), wash the culture medium once, and centrifuge again (to wash away the collagenase), so as to obtain skin fibroblasts.

[0025] The system for transforming skin fibroblasts into artificial intervertebral discs in this embodiment includes transforming growth factor β, artificial intervertebral disc cell culture medium and cell matrix construct polylactic acid.

[0026] Among them, the composition of artificial i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a system for transforming skin fibroblast cells into an artificial intervertebral disc and a using method thereof. The system comprises growth factors and artificial intervertebral disc cell culture fluid. Through the system, after being transformed into rtificial intervertebral disc cells, the skin fibroblast cells are injected into the intervertebral disc, development offibrosis of the intervertebral disc is promoted, accordingly, the height of vertebrae interval is kept, a signal of the intervertebral disc is improved, the aim of regulating and controlling degeneration of the intervertebral disc is achieved, and the intervertebral disc gains new mechanical strength.

Description

technical field [0001] The invention belongs to the field of intervertebral disc degeneration, and particularly relates to a system for converting skin fibroblasts into artificial intervertebral discs and a method for using the same. Background technique [0002] With the extension of human life expectancy, the acceleration of the pace of life, and the increase of bowing and bending work methods, the incidence of lumbar degenerative diseases is increasing. The degeneration of the lumbar intervertebral disc is the main cause of low back pain and radiating pain in the lower limbs. The intervertebral disc is the largest major avascular, anervous, and alymphatic structure in the human body. The intervertebral disc is critical to the normal function of the spine as it provides flexibility and mechanical stability in axial compression, flexion and extension. The intervertebral disc is composed of several specific connective tissues: 1. The hyaline cartilage of the cartilaginous ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/071
CPCC12N5/0602C12N2500/02C12N2501/15C12N2533/30
Inventor 赵杰陈辰周唐峻秦安张凯杜琳
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com