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Individualized medication gene testing reagent kit for beta-receptor blocker

A technology for gene detection and blockers, which is applied in the field of gene detection kits for individualized drug use of β-receptor blockers, and can solve problems such as no response, adverse drug reactions, and affecting treatment effects

Inactive Publication Date: 2019-01-04
中科基因生物科技(江苏)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Variations in the CYP2D6 gene can lead to adverse drug reactions or no response to drugs at normal drug doses, thereby affecting the therapeutic effect

Method used

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  • Individualized medication gene testing reagent kit for beta-receptor blocker
  • Individualized medication gene testing reagent kit for beta-receptor blocker
  • Individualized medication gene testing reagent kit for beta-receptor blocker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Prepare a gene detection kit for detecting β-receptor blockers for individualized drug use for one person, including the following components:

[0032] (1) 2 pairs of CYP2D6*10 and ADRB1 (1165G>C) site amplification and sequencing primers, each primer 1OD, and the primer sequences are shown in Table 1;

[0033] (2) PCR amplification reagent: 15 μL of Taq enzyme mixture (dNTP, 10×PCR reaction buffer, MgCl 2 );

[0034] (3) PCR product purification reagent: 3.2 μL SAP enzyme mixture (SAP enzyme, ExoI enzyme, deionized water);

[0035] (4) Sequencing reagents: 4.4 μL BigDye mix (Bigdye, 5×seq), 5 μL EDTA, 60 μL ethanol solution (100%), 200 μL ethanol solution (70%), 16 μL Hi-Di.

[0036] This kit is stored at -20°C, and repeated freezing and thawing should be avoided as much as possible.

Embodiment 2

[0038] The prepared one-person kit is used for genotyping detection of the gene for individualized drug use of β-blockers, including steps:

[0039] (1) extract the genomic DNA of the sample;

[0040] (2) PCR amplification of CYP2D6*10 and ADRB1 (1165G>C) loci

[0041] For CYP2D6*10 and ADRB1 (1165G>C) sites, design primers according to Table 1 for PCR amplification. Each reaction system has a total volume of 16 μL, including 7.5 μL of Taq enzyme mixture (dNTP, 10×PCR reaction buffer, MgCl 2 ), deionized water 5.5 μL, primer pair (10uM) 1 μL, genomic DNA (100ng / ul) 1ul. The reaction conditions are 95°C for 15 minutes, 14 cycles (-0.5°C / cycle) of 94°C for 30 seconds, 63°C for 30 seconds, and 72°C for 45 seconds; then 30 cycles of 94°C for 30 seconds and 56°C for 30 seconds , 72°C for 45 seconds, and finally 72°C for 10 minutes;

[0042] (3) Purification of PCR products

[0043] Each reaction has a total volume of 5.6 μL. Add 4 μL of PCR product, then add 1.6 μL of SAP enzy...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to an individualized medication gene testing reagent kit for a beta-receptor blocker. The individualized gene testingreagent kit for the beta-receptor blocker mainly comprises: (1) two pairs of CYP2D6*10 and ADRBB1(1165 G) [C] locus amplification and sequencing primers, (2) PCR amplification reagents, (3) a PCR product purification reagent, and (4) a DNA sequencing reagent. The reagent kit is used for detecting the loci of the beta-receptor blocker drug metabolizing enzyme and the receptor CYP2D6*10 and ADRBB1 (1165 G) [C] of the hypertension patient, uses the key technology for Sanger sequencing (the accuracy rate is more than 99.9%). The genotypes of the two loci are proved to be closely associated with the curative effect of medication of the beta-receptor blocker, so that the gene testing reagent kit can be used for guiding the hypertension patient in using the beta-receptor blocker drug reasonably and safely according to the testing result of the gene testing reagent kit.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a gene detection kit for detecting beta-receptor blockers for individualized medication. Background technique [0002] Statistics show that the prevalence of hypertension in my country is increasing year by year. By the end of 2014, the number of hypertensive patients in my country has reached 270 million, and the incidence rate is as high as 25.5%. However, the treatment rate is 41.1%, and the control rate is only 13.8%. The incidence of acute cardiovascular disease events, disability rates, and mortality rates have always remained high. Hypertension and related adverse events have become major public health problems that endanger the health of our people. Clinical drug results show that there are large individual differences in the efficacy and adverse reactions of hypertension medications. Although new drugs emerge in an endless stream, the blood pressure contro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/106C12Q2600/156
Inventor 马端姜昕徐沁张静景丹丹唐帅男
Owner 中科基因生物科技(江苏)有限公司
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