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Preparation method of micro column gel for high-quality blood type test cards

A technology of micro-column gel and detection card, which is applied in the field of preparation of micro-column gel for high-quality blood type detection card, and can solve the problems of poor specificity, low sensitivity, micro-column gel papers and patents, etc.

Active Publication Date: 2019-01-04
JIANGYIN LIBO MEDICINE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the microcolumn gel blood type card products in the market are mixed, and the core of the preparation of high-quality blood microcolumn gel card is the preparation of microcolumn gel. After searching, there is no research on the core technology of microcolumn gel. Papers and patents, and the micro-column gels on the market have poor specificity when screening free molecules, so the sensitivity is not high

Method used

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  • Preparation method of micro column gel for high-quality blood type test cards
  • Preparation method of micro column gel for high-quality blood type test cards
  • Preparation method of micro column gel for high-quality blood type test cards

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Embodiment Construction

[0022] Step 1. Screening of polyacrylamide dextran gel

[0023] The polyacrylamide dextran gel is selected, and the proportion of the gel particle size is 10-100 nanometers ≥ 65%. Every 1 gram of the gel is soaked in 20ml of normal saline for 24-48 hours to ensure that the gel is fully swollen.

[0024] Step 2, washing of polyacrylamide dextran gel

[0025] For polyacrylamide-dextran gels that are fully swollen, it is necessary to wash away the damaged gel fragments and fine particles in the solution. The specific method is as follows: the dextran gel swelling gel is thoroughly mixed, centrifuged with 900g centrifugal force for 2 minutes, and the supernatant is sucked off. Then add an equal volume of 0.9% sodium chloride solution, and repeat the operation 3-5 times until the upper layer has no fine particles.

[0026] Step 3. Soaking of polyacrylamide dextran gel

[0027] The gel soak formula is as follows:

[0028] Sodium chloride 2.5×10-3g / ml

[0029] PEG400 (2.2-2.6)×1...

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Abstract

The invention relates to a preparation method of micro column gel for high-quality blood type test cards. The preparation method includes: screening to select polyacrylamide sephadex, washing the polyacrylamide sephadex, soaking the polyacrylamide sephadex, and washing again the polyacrylamide sephadex. The preparation method herein helps prepare micro column gel for high-quality blood type test cards, and blood type test cards produced with the micro column gel prepared via the method can gain evidently improved sensitivity and specificity.

Description

technical field [0001] The invention relates to a preparation method of a microcolumn gel for a high-quality blood type detection card. Background technique [0002] A milestone event in the history of blood transfusion development was the discovery of A, B, and O blood types in 1900 by Karl Landersteiner, a scientist at the University of Vienna in Austria. Two years later, his students discovered the AB blood type, which ushered in a new era of safe blood transfusion, for which Landersteiner won the Nobel Prize in Medicine in 1930. From 1927 to 1947, the MNS, P and Rh systems were discovered successively, and the safe blood transfusion technology was initially established. [0003] Clinically effective use of blood first requires clinicians to clarify which patients need blood transfusion under what circumstances, effectively treat coagulation disorders and prevent adverse reactions of blood transfusion. Review the ABO blood type of the blood donor, and routinely check th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/80
CPCG01N33/80
Inventor 陈玉平陈芳芳王布强薄薇
Owner JIANGYIN LIBO MEDICINE BIOTECH