Nondestructive fluorescence detection spectrograph suitable for cell level

Abstract

The invention discloses a cell level nondestructive fluorescence detection spectrograph. The cell level nondestructive fluorescence detection spectrograph comprises an exciting light source; an exciting light probe is connected to the lower end of the exciting light source; the exciting light probe is connected with an exciting light position control system through an exciting light probe fixing device; a precision NC motor is arranged on the exciting light position control system; a sample cell for placing cell samples is formed under the exciting light probe; and a fluorescence spectrum collecting and analyzing system is under the sample cell. The sample cell is formed a sample cell position control system which is hollowed-out in the middle and fixed on the surrounding. The cell level nondestructive fluorescence detection spectrograph disclosed by the invention can be used for single cell/sub-cell fluorescence analysis, can realize phosphorescence life testing and can detect the change of fluorescence intensity with time within a time scale range from milliseconds to hours; and the cell level nondestructive fluorescence detection spectrograph disclosed by the invention can be used for single cell/sub-cell fluorescence microscopy imaging, micro-area fluorescence spectrum analysis and integral steady-state fluorescence spectrum analysis in the X-Y direction and has greater guiding significance in early cancer detection and screening.

Description

technical field [0001] The invention relates to the technical field of application of optical technology in the biological field. Background technique [0002] In recent years, with the gradual deepening of research on cell physiology, scientists have discovered that even with the same genetic material and the same surrounding environment, these cells will still develop in different directions, and sometimes produce different functions. Individual cells appear to be the same, but their chemical composition and stress response are very different, and changes in individual cells are also related to major diseases such as cancer and neurological diseases. Single-celled biological processes in plants and animals are also far from fully understood. Single-cell analysis is therefore an important means of understanding and deciphering many critical life processes. However, the size of a single cell is very small, and the chemical components contained in it are also ultra-trace. H...

AI Technical Summary

Problems solved by technology

However, the size of a single cell is very small, and the chemical components contained in it are also ultra-trace. However, the current analysis method Western blot needs to break millions of cells to achieve the detection of intracellular biochemical indicators. Therefore, traditional analysis Method is insufficient to detect differences at the level of individual cells

Not only that, the traditional methods are mostly fixed-point detection, which cannot realize real-time monitoring

[0003] The current fluorescence spectrometers are generally used for the detection of macroscopic samples, an

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