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Tissue culture method for Polygonatum cyrtonema

A Polygonatum polyflora, tissue culture technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems that there is no method for tissue culture and rapid propagation of Polygonatum polyflora tubers, meet the requirements of rapid and efficient production, and shorten emergence Time, the effect of increasing the emergence rate

Active Publication Date: 2019-01-11
重庆市药物种植研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is no tissue culture and rapid propagation method for Polygonatum multiflora tuber at present

Method used

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  • Tissue culture method for Polygonatum cyrtonema
  • Tissue culture method for Polygonatum cyrtonema

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Embodiment 1 Polygonatum polyflora tissue culture medium 1

[0066] Explant treatment medium: add 6-benzylaminoadenine 2.0mg / L, sucrose 25g / L and agar 6g / L on the basis of MS medium;

[0067] Callus induction medium: on the basis of MS medium, add 6-benzylaminoadenine 2.0mg / L, sucrose 25g / L and agar 6g / L;

[0068] Tuber induction medium: 0.5 mg / L naphthaleneacetic acid, 25 g / L sucrose and 6 g / L agar were added on the basis of 1 / 2 MS medium.

Embodiment 2

[0069] Embodiment 2 Polygonatum polyflora tissue culture medium 2

[0070] Explant treatment medium: add 6-benzylaminoadenine 2.5mg / L, sucrose 20g / L and agar 6g / L on the basis of MS medium;

[0071] Callus induction medium: on the basis of MS medium, add 6-benzylaminoadenine 3.0mg / L, sucrose 25g / L and agar 6g / L;

[0072] Tuber induction medium: 0.3mg / L naphthaleneacetic acid, 25g / L sucrose and 6g / L agar were added on the basis of 1 / 2MS medium.

Embodiment 3

[0073] Embodiment 3 Polygonatum polyflora tissue culture medium 3

[0074] Explant treatment medium: add 6-benzylaminoadenine 3.0mg / L, sucrose 20g / L and agar 6g / L on the basis of MS medium;

[0075] Callus induction medium: on the basis of MS medium, add 6-benzylaminoadenine 3.0mg / L, sucrose 25g / L and agar 6g / L;

[0076] Tuber induction medium: 0.2mg / L naphthaleneacetic acid, 25g / L sucrose and 6g / L agar were added on the basis of 1 / 2MS medium.

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Abstract

The invention belongs to the field of medicament plantation, and concretely relates to a tissue culture method for Polygonatum cyrtonema. The invention also provides a composition for tissue culture of the Polygonatum cyrtonema, and a method for culturing the Polygonatum cyrtonema by using the composition. The method meets rapid and efficient Polygonatum cyrtonema seedling production requirementsby adopting young tubers of the Polygonatum cyrtonema as a material, adopting a plant tissue culturing technology, inducing calluses by the Polygonatum cyrtonema tubers, proliferating and differentiating the calluses and breaking the dormancy of Polygonatum cyrtonema buds through low-temperature treatment, and is suitable for large-scale production of the Polygonatum cyrtonema seedlings.

Description

technical field [0001] The invention belongs to the field of medicine planting, and in particular relates to a method for culturing Polygonatum polyflora tissue. Background technique [0002] Polygonatum cyrtonema is a Polygonatum plant of Liliaceae Polygonatum genus Polygonatum cyrtonema, Polygonatum sibiricum and Polygonatum kingianum coll. The rhizome of Polygonatum Polyflora has the traditional effects of invigorating qi and nourishing yin, invigorating the spleen, nourishing the lungs, and benefiting the kidneys. Soreness of the waist and knees, etc. Polygonatum multiflora has broad application prospects in anti-aging, regulating immunity, regulating blood lipids, improving memory, anti-tumor, and antibacterial. Polygonatum multiflora is distributed in Chongqing, Sichuan, Hunan, Hubei, Guizhou, Zhejiang and other provinces. [0003] The traditional propagation methods of Polygonatum multiflora include tuber and seed propagation. The tuber propagation of Polygonatum ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 韩量肖波杨天建张万超李娜雷美艳
Owner 重庆市药物种植研究所
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