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A kind of human leukocyte hla-b*27 genotyping primer set and application

A technology of HLA-B and genotyping, applied in the direction of recombinant DNA technology, biochemical equipment and methods, microbial determination/testing, etc.

Active Publication Date: 2021-10-22
江苏中济万泰生物医药有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to overcome the defect that the correctness of the existing HLA-B*27 detection technology needs to be further improved, and to provide a human leukocyte HLA-B*27 genotyping primer set and its application

Method used

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Embodiment

[0020] A human leukocyte HLA-B*27 genotyping primer set, including allele primer pairs LB27TF and LB27TR for detecting blood types B27T, B2705, B2704 and B2706; LB2705F and LB2705R; LB2706F1 and LB2706R1; LB2706F2 and LB2706R2 and internal reference primers right;

[0021] The sequences of the LB27TF and LB27TR are GTGGGCTACGTGGACGACACGCTGTTC and GTCAGTCTGTGCCTTGGCCTTGCAGATC respectively;

[0022] The sequences of LB2705F and LB2705R are GGCTCCCACTCCATGAGGTATTTCCA and CGGAGCAGGGTCCGCAGGTCCTC, respectively;

[0023] The sequences of the LB2706F1 and LB2706R1 are CCAGGGTCTCACACCCTCCAGAATATGT and TAGGCTCTCAGCTGCTCCGCCACA, respectively;

[0024] The sequences of LB2706F2 and LB2706R2 are CAGGGTCTCACACCCTCCAGAATATGTA and TTGCCGTCGTAGGCGTCCTGGTCATAC respectively.

[0025] Further, the sequences of the internal reference primer pair are GTCCTGGAGGAGAAGAGGA and ATTGAGGACCCTCTGTGTAT respectively.

[0026] The above primers can be used in the preparation of products for detecting HLA...

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Abstract

The invention discloses a human leukocyte HLA‑B*27 genotyping primer set, which includes detecting blood types A and A respectively. 205 , B and O (261G缺失) , O 1 , O 2 The allelic primer pair AR and AF; A 2 R and A 2 F;BR and BF;0 T R and O T F;O 1 R and O 1 F;O 2 R and O 2 F and internal reference primer pair; the allelic primer set designed by the present invention has high power and strong specificity, and the internal reference primer pair internal reference 1R and internal reference 1F, internal reference 2R and internal reference 2F are designed according to the conserved fragment of human albumin gene, and each detection The number of pairs of allelic primers in the well is 2 to 4 pairs, and at the same time, it contains 2 pairs of internal references to ensure the quality and correctness of PCR amplification, thereby ensuring the reliability of the test results.

Description

technical field [0001] The invention relates to the technical field of gene diagnosis products, in particular to a human leukocyte HLA-B*27 genotyping primer set and its application. Background technique [0002] HLA (human leukocyte antigen), that is, human leukocyte antigen is an important immune genetic system of the human body, and it is also the most complex polymorphic system known to the human body. As an antigen, HLA stimulates the body to produce the properties or molecular structure of corresponding antibodies, which is the main cause of rejection in organ transplantation and the basis for serological and cytological matching. HLA has class I, class II, and class III genes, and HLA-B* is one of the three loci (HLA-A*, HLA-B*, HLA-C*) of class I HLA genes. The HLA-B27 gene is the allele on the B site of the human HLA-I class molecule, located on the short bamboo of chromosome 6, organized by 8 exons and 7 introns, encoding a protein with a molecular mass of 43KD g...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/166
Inventor 林裕翔吴明磊朱晓洁
Owner 江苏中济万泰生物医药有限公司
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