A method for determining the role of the methylation site change of potato StALDH gene in low temperature stress

A technology of low temperature stress and methylation, applied in the field of genetic engineering, can solve problems that have not been studied in depth

Inactive Publication Date: 2019-01-11
INNER MONGOLIA UNIV OF SCI & TECH
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Problems solved by technology

But so far, there are few reports on how the methylation sites of the ALDH gene change in potato under low temperature stress, how the gene expression level changes after the methylation level of the gene changes, how the methylation changes respond to low temperature stress and What is the tolerance to low temperature stress after changes in gene methylation levels, these issues have not been studied in depth

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  • A method for determining the role of the methylation site change of potato StALDH gene in low temperature stress
  • A method for determining the role of the methylation site change of potato StALDH gene in low temperature stress
  • A method for determining the role of the methylation site change of potato StALDH gene in low temperature stress

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Embodiment

[0024] Provided in this embodiment is a method for determining the effect of changes in the methylation sites of the potato StALDH gene in low temperature stress (such as figure 1 shown), the specific steps are as follows.

[0025] (1) Analysis of changes in DNA methylation sites of StALDH gene under low temperature stress

[0026]Extract the "Atlantic" genomic DNA that was treated at 4°C for 0h, 48h, and 60h, and carry out DNA sulfite conversion treatment on the above samples with a sulfite conversion kit, and the unmethylated cytosine (C) was converted into urine pyrimidine (U), while methylated cytosine (C) remains unchanged. Four types of samples to be tested were obtained: A low temperature untreated-sulfite unconverted (Original), B low temperature untreated-sulfite converted (Original-BST), C low temperature treated for 48h-sulfite converted (48H-BST) , D low temperature treatment 60h-sulfite conversion (60H-BST).

[0027] Using the above sample as a template, the St...

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Abstract

The invention relates to a method for determining the role of the methylation site change of potato StALDH gene in low temperature stress, which includes the analysis of DNA methylation site of StALDHgene under low temperature stress, the analysis of expression level of StALDH gene under low temperature stress and the analysis of NbALDH function in plant development and response to low temperature stress. A low temperature stress response mechanism of potato is study from epigenetics field, A method for isolate genes invol in response to low temperature stress and changes of their methylationsites was developed, Therefore, cultivating potato varieties with cold tolerance and freeze resistance can resist the adverse effects of sudden low temperature on potato production, broaden the research on potato tolerance to low temperature stress, and have very important theoretical basis and practical guiding significance for ensuring high yield and stable harvest of potato in China. At the same time, it provides a theoretical reference for the role of DNA methylation modification in the response of other plants to stress.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a method for determining the role of the methylation site change of the potato StALDH gene in low temperature stress. Background technique [0002] Potatoes are the third most important crop in the world, after wheat and rice. Under the influence of population growth, reduction of arable land area, and water shortage, there is a potential crisis in my country's food security. The continuous development of the potato industry will play an important role in alleviating the pressure on my country's food safety. my country is a big potato producing country, accounting for 1 / 4 of the world's potato planting area and 1 / 5 of the output, playing a decisive role in the world. Inner Mongolia Autonomous Region is the largest potato production area in my country. Due to its unique climatic conditions, it has become the main production area for seed potato and commercial potato ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/82A01H5/00A01H6/82
CPCC12N15/8218C12N15/8273C12Q1/6895C12Q2600/13C12Q2600/154C12Q2600/158
Inventor 辛翠花郭江波池俊玲赵慧孙玮贺引弟
Owner INNER MONGOLIA UNIV OF SCI & TECH
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