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Rapid Breeding Method of Sargassum valsonii

A rapid technology of Sargassum varnishae, which is applied in the field of rapid breeding of Sargassum varnishae, can solve the problems of inability to rapidly and mass-produce breeding, long culturing time, and high cost, and achieves rapid breeding, short culturing time, and low cost. Effect

Active Publication Date: 2021-11-09
杭州园泰生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The document CN103202214A discloses a breeding method of Sargassum varschii, which not only effectively controls and prolongs the sexual reproduction time, but the fertilization rate reaches more than 95%, but the method is easily limited by objective conditions such as culture conditions and the amount of eggs laid , unable to reproduce rapidly and in large quantities
Document CN103385170A discloses a method for raising seedlings by tissue culture of Sargassum waschii, in which leaves of young seedlings and rhizoids of high-day-old seedlings are used for section tissue culture to obtain regenerated seedlings. This method is relatively easy to control and can obtain a larger number of seedlings, but The cultivation time is longer, about 60 days, and the process is more complicated and the cost is higher

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  • Rapid Breeding Method of Sargassum valsonii

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Effect test

Embodiment 1

[0026] The rapid breeding method of Sargassum valsonii comprises the steps:

[0027] Select healthy Sargassum varsleyi seedlings (30-40 days old), collect the leaves, soak them in 10g / L potassium iodide aqueous solution for 20min, take them out, then place them in a tissue grinder, and process them at a speed of 10000rpm for 3min , to obtain tiny tissue pieces containing 10-50 cells, put the tiny tissue pieces in the culture medium, the culture conditions are: temperature 22°C, light intensity 3000lux, light-dark ratio 12:12; culture for 10d, then replace the culture medium , then add GA3 (gibberellin) and photosynthetic bacteria, control the concentration of GA3 in the culture medium to 30mg / L, and the concentration of photosynthetic bacteria to 1×10 8 cfu / L, the culture conditions are: temperature 19°C, light intensity 3000lux, light-to-dark ratio 10:14, when the length of the main leaf reaches 5mm or more, the breeding process is completed;

[0028] The photosynthetic bact...

Embodiment 2

[0030] The rapid breeding method of Sargassum valsonii comprises the steps:

[0031] Select healthy Sargassum varsleyi seedlings (30-40 days old), collect the leaves, soak them in 10g / L potassium iodide aqueous solution for 20min, take them out, then place them in a tissue grinder, and process them at a speed of 10000rpm for 5min , to obtain tiny tissue pieces containing 10-50 cells, put the tiny tissue pieces in the culture medium, the culture conditions are: temperature 23°C, light intensity 4000lux, light-dark ratio 12:12; culture for 10d, then replace the culture medium , then add GA3 (gibberellin) and photosynthetic bacteria, control the concentration of GA3 in the culture medium to 50mg / L, and the concentration of photosynthetic bacteria to 2×10 8cfu / L, the culture conditions are: temperature 20°C, light intensity 2000lux, light-to-dark ratio 10:14, when the length of the main leaf reaches 5mm or more, the breeding process is completed;

[0032] The photosynthetic bacte...

Embodiment 3

[0034] The rapid breeding method of Sargassum valsonii comprises the steps:

[0035] Select healthy Sargassum varsleyi seedlings (30-40 days old), collect leaves, soak in 10g / L potassium iodide aqueous solution for 30min, take them out, then place them in a tissue grinder, and process them at 10000rpm for 4min , to obtain tiny tissue pieces containing 10-50 cells, put the tiny tissue pieces in the culture medium, the culture conditions are: temperature 22°C, light intensity 3000lux, light-dark ratio 12:12; culture for 10d, then replace the culture medium , then add GA3 (gibberellin) and photosynthetic bacteria, control the concentration of GA3 in the culture medium to 40mg / L, and the concentration of photosynthetic bacteria to 3×10 8 cfu / L, the culture conditions are: temperature 20°C, light intensity 2000lux, light-to-dark ratio 10:14, when the length of the main leaf reaches 5mm or more, the breeding process is completed;

[0036] Described photosynthetic bacterium selects ...

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Abstract

The invention belongs to the technical field of algae cultivation, and discloses a rapid breeding method for Sargassum varschii, which comprises the following steps: step 1) selecting healthy Sargassum varschii seedlings, collecting leaves, soaking them in potassium iodide aqueous solution for 10-30 minutes, and taking them out , and then placed in a tissue masher, processed at a speed of 10,000rpm for 3-5min to obtain a tiny tissue block containing 10-50 cells, which was placed in the culture medium and incubated for 10 days; step 2) replace with a new one Add GA3 and photosynthetic bacteria to the culture solution, and the breeding process is completed when the length of the main leaf blade reaches more than 5mm. The method of the invention can increase the breeding rate of the sargassum varschii, has low cost, is easy to operate, and can realize artificial large-scale cultivation in a relatively short period of time.

Description

technical field [0001] The invention belongs to the technical field of algae cultivation, and in particular relates to a rapid breeding method for Sargassum varschii. Background technique [0002] Seaweed is an important part of marine plants, and macroalgae are important framework organisms in seaweed farms. Sargassum is the largest genus in Phaeophyta. Many species in this genus have important economic and ecological value, and they are also important members of coastal seaweed fields in China and Japan. Sargassum algae is large, grows rapidly, and has high economic value. In terms of industry and agriculture, Sargassum is an important raw material for extracting alginate, and can also be used as a high-quality fertilizer for crops. In terms of medicine, Sargassum can extract a variety of active substances, some of which have good anti-tumor effects. In the breeding industry, Sargassum can be used as a natural high-quality feed for a variety of economic animals, and has...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 张菊萍董国强胡叶飞金荣培张宏杰
Owner 杭州园泰生物科技有限公司