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Scale culture and polysaccharide extraction method for sargassum vachellianum

A technology of Sargassum varnishae and process, which is applied in the field of large-scale cultivation and extraction of polysaccharides, can solve the problems of inability to rapidly and massively realize breeding, long cultivation time, and increased cost, and achieves the promotion of cell division, spore growth, and cultivation time. Short, increase the number of blades and the effect of blade length

Active Publication Date: 2019-02-12
杭州园泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The document CN103202214A discloses a breeding method of Sargassum varschii, which not only effectively controls and prolongs the sexual reproduction time, but the fertilization rate reaches more than 95%, but the method is easily limited by objective conditions such as culture conditions and the amount of eggs laid , unable to reproduce rapidly and in large quantities
Document CN103385170A discloses a method for raising seedlings by tissue culture of Sargassum waschii, in which leaves of young seedlings and rhizoids of high-day-old seedlings are used for section tissue culture to obtain regenerated seedlings. This method is relatively easy to control and can obtain a larger number of seedlings, but The cultivation time is longer, about 60 days, and the process is more complicated and the cost is higher

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  • Scale culture and polysaccharide extraction method for sargassum vachellianum

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Effect test

Embodiment 1

[0032] The technique for the large-scale cultivation of Sargassum waschii and the extraction of polysaccharides comprises the following steps:

[0033]Select healthy Sargassum varsleyi seedlings (30-40 days old), collect the leaves, soak them in 10g / L potassium iodide aqueous solution for 20min, take them out, then place them in a tissue grinder, and process them at a speed of 10000rpm for 3min , to obtain tiny tissue pieces containing 10-50 cells, put the tiny tissue pieces in the culture medium, the culture conditions are: temperature 22°C, light intensity 3000lux, light-dark ratio 12:12; culture for 10d, then replace the culture medium , then add GA3 (gibberellin) and photosynthetic bacteria, control the concentration of GA3 in the culture medium to 30mg / L, and the concentration of photosynthetic bacteria to 1×10 8 cfu / L, the culture conditions are: temperature 19°C, light intensity 3000lux, light-to-dark ratio 10:14, when the length of the main leaf blade reaches more than...

Embodiment 2

[0037] The technique for the large-scale cultivation of Sargassum waschii and the extraction of polysaccharides comprises the following steps:

[0038] Select healthy Sargassum varsleyi seedlings (30-40 days old), collect the leaves, soak them in 10g / L potassium iodide aqueous solution for 20min, take them out, then place them in a tissue grinder, and process them at a speed of 10000rpm for 5min , to obtain tiny tissue pieces containing 10-50 cells, put the tiny tissue pieces in the culture medium, the culture conditions are: temperature 23°C, light intensity 4000lux, light-dark ratio 12:12; culture for 10d, then replace the culture medium , then add GA3 (gibberellin) and photosynthetic bacteria, control the concentration of GA3 in the culture medium to 50mg / L, and the concentration of photosynthetic bacteria to 2×10 8 cfu / L, the culture conditions are: temperature 20°C, light intensity 2000lux, light-to-dark ratio 10:14, when the length of the main leaf leaves reaches more th...

Embodiment 3

[0042] The technique for the large-scale cultivation of Sargassum waschii and the extraction of polysaccharides comprises the following steps:

[0043] Select healthy Sargassum varsleyi seedlings (30-40 days old), collect leaves, soak in 10g / L potassium iodide aqueous solution for 30min, take them out, then place them in a tissue grinder, and process them at 10000rpm for 4min , to obtain tiny tissue pieces containing 10-50 cells, put the tiny tissue pieces in the culture medium, the culture conditions are: temperature 22°C, light intensity 3000lux, light-dark ratio 12:12; culture for 10d, then replace the culture medium , then add GA3 (gibberellin) and photosynthetic bacteria, control the concentration of GA3 in the culture medium to 40mg / L, and the concentration of photosynthetic bacteria to 3×10 8 cfu / L, the culture conditions are: temperature 20°C, light intensity 2000lux, light-to-dark ratio 10:14, when the length of the main leaf blade reaches more than 5mm, the breeding ...

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Abstract

The invention belongs to the technical field of algae culture, and discloses a scale culture and polysaccharide extraction method for sargassum vachellianum. The scale culture and polysaccharide extraction method for the sargassum vachellianum comprises the following steps of: S1, tissue breeding; S2, scale culture; S3, crude polysaccharide extraction. According to the invention, the sargassum vachellianum is subjected to scale culture, biomass is improved, polysaccharide is extracted, and the method has good industrial value.

Description

technical field [0001] The invention belongs to the technical field of algae cultivation and polysaccharide extraction, and in particular relates to a process for large-scale cultivation of Sargassum varschii and extraction of polysaccharides. Background technique [0002] Seaweed is an important part of marine plants, and macroalgae are important framework organisms in seaweed farms. Sargassum is the largest genus in Phaeophyta. Many species in this genus have important economic and ecological value, and they are also important members of coastal seaweed fields in China and Japan. Sargassum algae is large, grows rapidly, and has high economic value. In terms of industry and agriculture, Sargassum is an important raw material for extracting alginate, and can also be used as a high-quality fertilizer for crops. In terms of medicine, Sargassum can extract a variety of active substances, some of which have good anti-tumor effects. In the breeding industry, Sargassum can be u...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P19/04C12R1/89
CPCC12N1/12C12P19/04
Inventor 张菊萍董国强胡叶飞金荣培张宏杰
Owner 杭州园泰生物科技有限公司