A method for cell immobilization of Thiobacillus thiotrophicus
A Thiobacillus thiophane, immobilized carrier technology, applied in microorganism-based methods, biochemical equipment and methods, immobilized on/in organic carriers, etc., can solve poor stability, application limitations, low mechanical strength, etc. problem, to achieve the effect of high stability, improved immobilization efficiency and effect, and low cost
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Embodiment 1
[0030] Put Thiobacillus thioexans CGMCC No.12756 in the culture medium (composition: NH 4 Cl 0.4g / L, MgSO 4 0.7g / L, K 2 HPO 4 4g / L, KH 2 PO 4 4g / L, Na 2 S 2 o 3 9g / L, pH 6.5), temperature 28°C, speed 160rpm / min after culturing for 48h, prepare a bacterial suspension with a cell concentration of 5g / L; The mass percentage in the solution is 3%, and at the same time, according to the volume ratio of the mixed solution to the polyethyleneimine solution 3%, a crosslinking agent polyethyleneimine with a mass concentration of 3% is added, and the mixed solution is obtained after mixing evenly; the obtained mixed solution is added to the mass Calcium chloride solution with a percentage concentration of 7%, wherein the mixed solution and calcium chloride solution are mixed at a volume ratio of 1:1, fully mixed, left standing at 20°C, hardened for 24 hours, filtered and washed to obtain immobilized cells. Take 1g of the resulting immobilized cells, add 10mL 5mol / L Na 2 In t...
Embodiment 2
[0033] Put Thiobacillus thioexans CGMCC No.12756 in the culture medium (composition: NH 4 Cl 0.5g / L, MgSO 4 0.8g / L, K 2 HPO 4 5g / L, KH 2 PO 4 5g / L, Na 2 S 2 o 3 10g / L, pH 6.8) After culturing for 60 hours at a temperature of 29°C and a rotational speed of 170rpm / min, a bacterial suspension with a bacterial cell concentration of 8g / L was prepared; then a certain amount of carrier carrageenan was added to make the carrageenan in the mixture The mass percentage is 4%, and at the same time, according to the volume ratio of the mixed solution and the polyethyleneimine solution 3.5%, the crosslinking agent polyethyleneimine with a mass concentration of 5% is added, and the mixed solution is obtained after mixing evenly; the obtained mixed solution is added to the mass percent Calcium chloride solution with a concentration of 5%, in which the mixed solution and calcium chloride solution are mixed at a volume ratio of 1:2, fully mixed and left at 18°C, hardened for 20 hours...
Embodiment 3
[0036] Put Thiobacillus thioexans CGMCC No.12756 in the culture medium (composition: NH 4 Cl 0.6g / L, MgSO 4 0.9g / L,K 2 HPO 4 6g / L, KH 2 PO 4 6g / L, Na 2 S 2 o 312g / L, pH 7) After culturing for 72 hours at a temperature of 30°C and a rotational speed of 180rpm / min, a bacterial suspension with a cell concentration of 10g / L was prepared; then a certain amount of carrier carrageenan was added to make the carrageenan in the mixture The mass percentage is 5%, and at the same time, according to the volume ratio of the mixed solution and the polyethyleneimine solution 4%, the crosslinking agent polyethyleneimine with a mass concentration of 7% is added, and the mixed solution is obtained after mixing; the obtained mixed solution is added to the mass percent Calcium chloride solution with a concentration of 2%, wherein the mixed solution and calcium chloride solution are mixed at a volume ratio of 1:3.5, fully mixed, left standing at 15°C, hardened for 10 hours, filtered and w...
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