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A lung cancer screening kit

A reagent and technology for lung cancer, applied in the field of lung cancer screening kits, can solve the problems of missed cure opportunity and low survival rate, and achieve the effect of good clinical application prospects.

Active Publication Date: 2019-08-06
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At the same time, lung cancer is a disease that is good at hiding. It often shows clinical symptoms when the disease develops to an advanced stage. 70-80% of lung cancer patients are already in the middle or late stage when they are diagnosed with lung cancer symptoms. The best time to cure, the five-year survival rate is low

Method used

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  • A lung cancer screening kit
  • A lung cancer screening kit
  • A lung cancer screening kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 The relationship between the expression level of non-phosphorylated SRMS and lung cancer

[0022] 1. Experimental method

[0023] 1. Clinical data

[0024] 37 cases of paraffin sections of lung cancer patients and 30 cases of remote controls (referring to paraffin sections of distal normal lung tissue of lung cancer patients, 5 cm away from cancer tissue, and pathologically confirmed as normal tissue) were selected. The basic information is shown in Table 1.

[0025] Table 1 Basic clinical information

[0026]

[0027] * Note: Others include large cell carcinoma, adenosquamous carcinoma, and small cell carcinoma.

[0028] 2. Detection of the expression level of non-phosphorylated SRMS

[0029] Immunohistochemistry (IHC) using SRMS as an index was performed on the paraffin sections of lung cancer patients and the control paraffin sections of remote tissues.

[0030] Non-phosphorylated SRMS antibody: purchased from ProteinTech, USA, Cat. No. 26447-1-AP; ...

Embodiment 2

[0061] Example 2 The composition of the kit for detecting the expression level of non-phosphorylated SRMS in the present invention and its application method

[0062] 1. The composition of the immunohistochemical detection kit

[0063] Detection kit (50 copies):

[0064] components volume Anti-phosphorylated SRMS antibody 30μl (please provide) EnVision TM HRP secondary antibody

100μl Antibody Diluent 10ml DAB developer 10ml Antigen Retrieval Solution 500ml Wash Buffer *

500ml immunohistochemistry pen 1 stick

[0065] Note: This kit is calculated as 100 μl of various experimental reagents per slice, and the specific dosage can be appropriately increased or decreased according to the size of the tissue.

[0066] 2. How to use the kit

[0067] The lung tissue of the sample to be tested is prepared as a paraffin section as a test specimen, and the expression level of non-phosphorylated SRMS is detected accordin...

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Abstract

The invention discloses a lung cancer screening kit. The lung cancer screening kit comprises an optional reagent used for detecting the non-phosphorylated SRMS protein expression level. The inventionfurther discloses use of the reagent used for detecting the non-phosphorylated SRMS protein expression level in preparation of a reagent for lung cancer screening. According to the kit in the invention, the expression level of non-phosphorylated SRMS is detected; therefore, the lung cancer risk of a person to be detected can be judged; the kit can be used for auxiliary diagnosis of the clinical lung cancer; the effective basis is provided for a patient to adopt a relevant treatment measure or decision; and the clinical application prospect is good.

Description

technical field [0001] The invention relates to a lung cancer screening kit. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world. Its morbidity and mortality are increasing year by year. Currently, the incidence rate ranks first in the world, which seriously threatens human health and life. [0003] The etiology of lung cancer is complex, and it is generally believed that the influencing factors include: ① smoking; ② environmental pollution: such as smog, interior decoration; ③ unhealthy lifestyle: such as poor eating habits, high life pressure; Silicosis, chronic bronchitis; ⑤ Intrinsic factors in the human body: such as family inheritance, decreased immune function, endocrine dysfunction, etc. [0004] At the same time, lung cancer is a disease that is good at hiding. It often shows clinical symptoms when the disease develops to an advanced stage. 70-80% of lung cancer patients are already in the middle or late stage when they...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/68G01N33/58G01N33/535G01N33/532
CPCG01N33/532G01N33/535G01N33/57484G01N33/58G01N33/581G01N33/68G01N2800/12G01N2800/52
Inventor 张立步宏李为民李丽
Owner WEST CHINA HOSPITAL SICHUAN UNIV